Charles P G, Dowling J P, Fuller P J
Prince Henry's Institute of Medical Research, Prince Henry's Hospital, Melbourne, Australia.
Ren Physiol Biochem. 1992 Jan-Feb;15(1):10-5. doi: 10.1159/000173436.
Na-K-ATPase is a heterodimer of alpha- and beta-subunits often referred to as the 'sodium pump' responsible for the maintenance of cell volume and electric potential across cell membranes. In the present study we have used antisense RNA probes to localize these subunits in the rat kidney by in situ hybridization. alpha 1-Subunit and beta-subunit gene expression are highest in cortical distal convoluted tubules, and the thick ascending limb of the loops of Henle; high in some proximal convoluted tubules, and low in the collecting ducts and blood vessels. Expression of the alpha 11 and alpha 111 isoforms is very low or absent throughout the kidney. These results confirm and extend studies of Na-K-ATPase enzyme activity levels and immunocytochemical localization in the kidney.
钠钾ATP酶是一种由α亚基和β亚基组成的异二聚体，常被称为“钠泵”，负责维持细胞膜两侧的细胞体积和电势。在本研究中，我们使用反义RNA探针通过原位杂交在大鼠肾脏中定位这些亚基。α1亚基和β亚基基因表达在皮质远曲小管和髓袢升支粗段最高；在一些近曲小管中较高，在集合管和血管中较低。α11和α111亚型在整个肾脏中的表达非常低或不存在。这些结果证实并扩展了对肾脏中钠钾ATP酶活性水平和免疫细胞化学定位的研究。
