Sugiura T
Department of Ophthalmology, School of Medicine, Kobe University, Japan.
Nippon Ganka Gakkai Zasshi. 1992 Jan;96(1):57-66.
The ultrastructural localization of glycosaminoglycans (GAGs) in the developing human outflow apparatus was investigated. The aqueous outflow system from human eyes at 26th and 36th fetal week and 2 years of age was stained with ruthenium red to identify GAGs with the transmission electron microscope. Luminal surface of the inner wall of the Schlemm's canal, basal lamina of the endothelial cells, basal lamina-like material, amorphous substances and collagen fibrils in juxta-canalicular tissue were associated with ruthenium red-stainable material. The basal lamina of the endothelial cells of Schlemm's canal was stained less obviously in 2-year-old trabecular tissue. The composition of the ruthenium red-stainable material was determined by treatment of each tissue with streptomyces hyaluronidase, chondroitinase AC, and chondroitinase ABC respectively. Hyaluronic acid was identified in each ruthenium red-stainable extracellular component. Chondroitin sulfate was identified in all ruthenium red-stainable components except luminal surface of the canal. The presence of dermatan sulfate was confirmed in the amorphous components and collagen fibrils of juxta-canalicular tissue. The results suggest that GAGs in fetal trabecular tissue already contribute to the outflow resistance and that alterations of the pattern of GAGs may take place as development proceeds.
研究了糖胺聚糖（GAGs）在发育中的人类房水流出装置中的超微结构定位。对妊娠第26周、第36周以及2岁儿童的人眼房水流出系统用钌红进行染色，以通过透射电子显微镜鉴定GAGs。施莱姆管内壁的腔面、内皮细胞的基膜、类基膜物质、小管周组织中的无定形物质和胶原纤维均与钌红可染物质相关。在2岁的小梁组织中，施莱姆管内皮细胞的基膜染色不太明显。分别用链霉菌透明质酸酶、软骨素酶AC和软骨素酶ABC处理各组织，以确定钌红可染物质的组成。在每个钌红可染的细胞外成分中均鉴定出透明质酸。除管腔面外，在所有钌红可染成分中均鉴定出硫酸软骨素。在小管周组织的无定形成分和胶原纤维中证实存在硫酸皮肤素。结果表明，胎儿小梁组织中的GAGs已经对流出阻力有贡献，并且随着发育的进行，GAGs模式可能会发生改变。
