含有克隆的红球菌基因的大肠杆菌所产生的靛蓝相关色素的鉴定。
Identification of indigo-related pigments produced by Escherichia coli containing a cloned Rhodococcus gene.
作者信息
Hart S, Koch K R, Woods D R
机构信息
Department of Microbiology, University of Cape Town, South Africa.
出版信息
J Gen Microbiol. 1992 Jan;138(1):211-6. doi: 10.1099/00221287-138-1-211.
Pigments produced by Escherichia coli containing a cloned piece of DNA from Rhodococcus sp. ATCC 21145 were extracted in chloroform and separated into blue and pink components. Evidence from TLC, NMR spectroscopy, absorption spectrum analysis and solubility behaviour suggested that the blue pigment was indigo and the pink pigment was indirubin, a structural isomer of indigo. The proposed pathway for pigment production on LB agar involves the conversion of tryptophan to indole by tryptophanase of E. coli and the oxidation of indole to indigo by the product of the cloned Rhodococcus DNA insert.
含有来自红球菌属ATCC 21145的一段克隆DNA的大肠杆菌所产生的色素用氯仿提取，并分离成蓝色和粉色成分。薄层色谱法、核磁共振光谱法、吸收光谱分析和溶解性实验的结果表明，蓝色色素是靛蓝，粉色色素是靛玉红，靛玉红是靛蓝的结构异构体。在LB琼脂上产生色素的推测途径包括：大肠杆菌的色氨酸酶将色氨酸转化为吲哚，以及克隆的红球菌DNA插入片段的产物将吲哚氧化为靛蓝。
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