Ensley B D, Ratzkin B J, Osslund T D, Simon M J, Wackett L P, Gibson D T
Science. 1983 Oct 14;222(4620):167-9. doi: 10.1126/science.6353574.
A fragment of plasmid NAH7 from Pseudomonas putida PpG7 has been cloned and expressed in Escherichia coli HB101. Growth of the recombinant Escherichia coli in nutrient medium results in the formation of indigo. The production of this dye is increased in the presence of tryptophan or indole. Several bacteria that oxidize aromatic hydrocarbons to cis-dihydrodiols also oxidize indole to indigo. The results suggest that indigo formation is due to the combined activities of tryptophanase and naphthalene dioxygenase.
恶臭假单胞菌PpG7的质粒NAH7片段已在大肠杆菌HB101中克隆并表达。重组大肠杆菌在营养培养基中生长会形成靛蓝。在色氨酸或吲哚存在的情况下，这种染料的产量会增加。几种将芳香烃氧化为顺式二氢二醇的细菌也会将吲哚氧化为靛蓝。结果表明，靛蓝的形成是色氨酸酶和萘双加氧酶共同作用的结果。
