Stagner J I, Samols E, Koerker D J, Goodner C J
Research Service, Veterans Administration Medical Center, Louisville, Kentucky 40206.
Pancreas. 1992;7(1):26-9. doi: 10.1097/00006676-199201000-00005.
The cellular sequence of intraislet vascular perfusion has been shown to be important in the regulation of islet hormone secretion in the rat and dog islet. In order to test whether a B to A to D sequence of islet cellular perfusion is also present in a nonhuman primate, pancreata from the rhesus monkey, Macaca mulatta, were isolated and perfused in vitro in the presence and absence of anti-insulin gamma globulin. In the presence of the insulin antibody, efflux concentration of insulin decreased rapidly (-95 +/- 1.8%), whereas glucagon and somatostatin concentrations increased (111 +/- 28% and 239 +/- 38%, respectively). These results suggest the presence of a B-A-D cellular sequence of vascular perfusion within the monkey islet. The present results also strongly support the hypothesis that a B-A-D sequence of islet perfusion is important in the regulation of islet hormone secretion and further emphasize the central role of the B-cell in intraislet cellular interactions. The results also suggest that, despite differences in islet anatomy, a B-A-D order of islet cellular perfusion may be the preferred functional sequence among mammalian species.
胰岛内血管灌注的细胞顺序已被证明在调节大鼠和犬胰岛的激素分泌中起重要作用。为了测试胰岛细胞灌注的B→A→D顺序是否也存在于非人灵长类动物中，从恒河猴（猕猴）分离胰腺，并在有和没有抗胰岛素γ球蛋白的情况下进行体外灌注。在存在胰岛素抗体的情况下，胰岛素的流出浓度迅速下降（-95±1.8%），而胰高血糖素和生长抑素浓度增加（分别为111±28%和239±38%）。这些结果表明猴胰岛内存在血管灌注的B-A-D细胞顺序。目前的结果也有力地支持了这样的假设，即胰岛灌注的B-A-D顺序在调节胰岛激素分泌中很重要，并进一步强调了B细胞在胰岛细胞内相互作用中的核心作用。结果还表明，尽管胰岛解剖结构存在差异，但胰岛细胞灌注的B-A-D顺序可能是哺乳动物物种中首选的功能顺序。
