Baum C M, Weissman I L, Tsukamoto A S, Buckle A M, Peault B
SyStemix Inc., Palo Alto, CA 94303.
Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2804-8. doi: 10.1073/pnas.89.7.2804.
We have identified a rare (0.05-0.1%) subset of human fetal bone marrow cells that contains multipotent hematopoietic precursors. The population of human precursor cells that express Thy-1 and CD34 but no known lineage markers is enriched for clonogenic activity that establishes long-term, multilineage (myelomonocytic and B lymphoid) cultures on mouse marrow stromal lines. Further, the Thy-1+CD34+ subset that takes up little of the fluorescent mitochondrial dye rhodamine 123 contains virtually all the cells that establish long-term cultures. In human fetal thymus transplanted into SCID (severe combined immunodeficiency) mice, Thy-1+CD34+ fetal bone marrow cells differentiate into T lymphocytes. In two of nine cases, allogeneic Thy-1+CD34+ cells could engraft intact human fetal bone marrow grown in SCID mice, resulting in donor-derived myeloid and B cells. By extrapolation, the rare human Thy-1+Lin-CD34+ cell population contains pluripotent hematopoietic progenitors; we propose that it is highly enriched for candidate hematopoietic stem cells.
我们已经鉴定出人类胎儿骨髓细胞中一个罕见的（0.05 - 0.1%）亚群，该亚群包含多能造血前体细胞。表达Thy-1和CD34但不表达已知谱系标志物的人类前体细胞群体富含克隆活性，能够在小鼠骨髓基质系上建立长期、多谱系（髓单核细胞和B淋巴细胞）培养物。此外，几乎不摄取荧光线粒体染料罗丹明123的Thy-1⁺CD34⁺亚群实际上包含了所有能够建立长期培养物的细胞。在移植到SCID（严重联合免疫缺陷）小鼠体内 的人类胎儿胸腺中，Thy-1⁺CD34⁺胎儿骨髓细胞分化为T淋巴细胞。在9例中的2例中，同种异体Thy-1⁺CD34⁺细胞能够植入在SCID小鼠体内生长的完整人类胎儿骨髓中，产生供体来源的髓系细胞和B细胞。由此推断，罕见的人类Thy-1⁺Lin⁻CD34⁺细胞群体包含多能造血祖细胞；我们认为它高度富集了候选造血干细胞。
