Strässle M, Stark G
Fakultät für Biologie, Universität Konstanz, Fed. Rep. Germany.
Photochem Photobiol. 1992 Mar;55(3):461-3. doi: 10.1111/j.1751-1097.1992.tb04262.x.
The ion channel formed by the peptide gramicidin A in planar lipid membranes is inactivated by visible light in the presence of the photosensitizer Rose Bengal. This is concluded from the strong decrease of the membrane conductance by more than two orders of magnitude. Experiments performed at different oxygen concentrations, in the presence of the singlet oxygen quenchers beta-carotene or alpha-tocopherol indicate, that presumably a type I process between the dye Rose Bengal and the tryptophan residues of the gramicidin channel with a subsequent oxidation of the tryptophans is responsible for the loss of the conductance properties of the channel.
在平面脂质膜中，肽短杆菌肽A形成的离子通道在存在光敏剂孟加拉玫瑰红的情况下会被可见光灭活。这是从膜电导强烈下降超过两个数量级得出的结论。在不同氧气浓度下，在单线态氧淬灭剂β-胡萝卜素或α-生育酚存在的情况下进行的实验表明，大概是染料孟加拉玫瑰红与短杆菌肽通道的色氨酸残基之间的I型过程以及随后色氨酸的氧化导致了通道电导特性的丧失。
