从人增生前列腺中对3α-和3β-羟基类固醇脱氢酶进行部分纯化。两种酶之间的比较。
Partial purification of 3 alpha- and 3 beta-hydroxysteroid dehydrogenases from human hyperplastic prostate. Comparison between the two enzymes.
作者信息
Amet Y, Simon B, Quemener E, Mangin P, Floch H H, Abalain J H
机构信息
Laboratoire de Biochimie, Faculté de Médecine, CHU Morvan, Brest, France.
出版信息
J Steroid Biochem Mol Biol. 1992 Mar;41(3-8):689-92. doi: 10.1016/0960-0760(92)90405-8.
3 alpha-Hydroxysteroid dehydrogenase (3 alpha-HSD) activity has been purified to homogeneity, the enzyme is a monomer with a Mw of 32,000 Da. 3 beta-Hydroxysteroid dehydrogenase (3 beta-HSD) activity has been partially purified and has an apparent Mw of 30,000 Da. Both enzymes have the same cofactor requirements, optimal pH. However, 3 beta-HSD appeared to be an integral protein dependent on protein environment for its activity while 3 alpha-HSD activity is a protein more loosely associated to membranes.
3α-羟基类固醇脱氢酶（3α-HSD）活性已被纯化至同质，该酶是一种分子量为32,000道尔顿的单体。3β-羟基类固醇脱氢酶（3β-HSD）活性已被部分纯化，其表观分子量为30,000道尔顿。两种酶具有相同的辅因子需求和最佳pH值。然而，3β-HSD似乎是一种依赖蛋白质环境发挥活性的整合蛋白，而3α-HSD活性是一种与膜结合较松散的蛋白质。
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