Caldera L H, Leon-Ponte M, Acquatella G, Bianco N E, Blanca I
Clinical Immunology Centre, University Hospital, Venezuela.
Clin Exp Immunol. 1992 Apr;88(1):143-8. doi: 10.1111/j.1365-2249.1992.tb03054.x.
Natural killer (NK) cytotoxic activity was simultaneously investigated in bone marrow mononuclear cells (BMMC) and peripheral blood lymphocytes (PBL) from nine Hodgkin's disease (HD) and 15 non-Hodgkin lymphoma (NHL) untreated patients. Twenty-five PBL samples and seven bone marrow specimens from healthy individuals were also included as control group (C). NK cell activity was evaluated in basal condition and post-stimulation with human recombinant IL-2 (rIL-2). Data were expressed in K values (number of BMMC or PBL needed to lyse 50% of the target cells). In basal condition, both HD and NHL patients showed a NK cell activity comparable to the C group, both in BMMC (HD, K = 2.48 +/- 1.3; NHL, K = 3.8 +/- 2.0; C, K = 3.2 +/- 0.7) and PBL (HD, K = 2.0 +/- 1.0; NHL, K = 2.3 +/- 1.0; C, K = 2.2 +/- 0.2). Stimulation with rIL-2 induced a significant and comparable enhancement of the NK activity in PBL from HD, NHL and C while the response to rIL-2 of the BMMC in most of the HD and NHL patients was significantly greater than the C group. Responder cells were characterized by negative selection with specific MoAb plus complement as a CD3-, CD16+, CD56+ cytotoxic cell and further confirmed by flow cytometry. We postulate that IL-2 activation of bone marrow NK cell precursors, in addition to enhancing the activity of circulating NK, may be of value for the therapeutic rationale of IL-2 in patients with lymphoma.
对9例未经治疗的霍奇金病（HD）患者和15例非霍奇金淋巴瘤（NHL）患者的骨髓单个核细胞（BMMC）和外周血淋巴细胞（PBL）同时进行自然杀伤（NK）细胞毒性活性研究。另外纳入25份健康个体的PBL样本和7份骨髓标本作为对照组（C）。在基础状态和用人重组白细胞介素-2（rIL-2）刺激后评估NK细胞活性。数据以K值表示（裂解50%靶细胞所需的BMMC或PBL数量）。在基础状态下，HD和NHL患者在BMMC（HD，K = 2.48±1.3；NHL，K = 3.8±2.0；C，K = 3.2±0.7）和PBL（HD，K = 2.0±1.0；NHL，K = 2.3±1.0；C，K = 2.2±0.2）中的NK细胞活性均与C组相当。用rIL-2刺激后，HD、NHL和C组PBL中的NK活性均显著且同等增强，而大多数HD和NHL患者BMMC对rIL-2的反应显著大于C组。通过用特异性单克隆抗体加补体进行阴性选择，将反应细胞鉴定为CD3 -、CD16 +、CD56 +细胞毒性细胞，并通过流式细胞术进一步确认。我们推测，骨髓NK细胞前体的IL-2激活除增强循环NK细胞的活性外，可能对淋巴瘤患者IL-2的治疗原理具有重要意义。
