用于测定血浆中拉西地平的高效液相色谱 - 放射免疫分析方法的验证
Validation of a high-performance liquid chromatographic-radioimmunoassay method for the determination of lacidipine in plasma.
作者信息
Pellegatti M, Braggio S, Sartori S, Franceschetti F, Bolelli G F
机构信息
Research Laboratories, Glaxo S.p.A., Verona, Italy.
出版信息
J Chromatogr. 1992 Jan 3;573(1):105-11. doi: 10.1016/0378-4347(92)80481-5.
A sensitive and reproducible method for the determination of lacidipine, a new potent antihypertensive dihydropyridine, is reported. The method involves solid-phase extraction, reversed-phase high-performance liquid chromatography and radioimmunoassay of the collected fraction. The assay provides a limit of detection of 20 pg/ml of plasma, allowing the determination of trough (24 h) plasma concentrations. The method is suitable for pharmacokinetic studies in man.
报道了一种灵敏且可重复的测定新型强效抗高血压二氢吡啶类药物拉西地平的方法。该方法包括固相萃取、反相高效液相色谱法以及对收集馏分的放射免疫测定。该测定法的检测限为血浆20 pg/ml，可用于测定谷值（24小时）血浆浓度。该方法适用于人体药代动力学研究。
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