Honkaniemi J, Pelto-Huikko M, Rechardt L, Isola J, Lammi A, Fuxe K, Gustafsson J A, Wikström A C, Hökfelt T
Department of Biomedical Sciences, University of Tampere, Finland.
Neuroendocrinology. 1992 Apr;55(4):451-9. doi: 10.1159/000126156.
The central amygdaloid nucleus (ACe) is part of the amygdaloid complex that participates in adrenocorticotrophin secretion, stress-related reactions and behavioral functions. The ACe contains numerous glucocorticoid receptor (GR)-immunoreactive (IR) neurons, and in addition it has been shown to contain several neuropeptide-IR somata and nerve terminals. In order to study the relationship between the GR- and neuropeptide-IR structures we mapped the distribution of GR-like immunoreactivity (LI) in amygdaloid complex and colocalized the neuropeptide- and GR-LIs in the ACe. In the amygdaloid complex the central, medial and cortical nuclei contained a high number of GR-IR neurons, whereas a moderate number of GR-IR neurons were observed in the basolateral and basomedial nuclei. Only a few GR-IR neurons were seen in the lateral nucleus. In the ACe, the majority of corticotrophin-releasing factor (CRF)-, met-enkephalin (met-ENK)-, neurotensin (NT)- and somatostatin (SOM)-IR neurons contained also GR-IR. About half of the substance P (SP)-IR neurons were seen to contain GR-IR, whereas only some of the few vasoactive intestinal polypeptide and cholecystokinin-IR neurons showed GR-LI. Nerve terminals containing calcitonin gene-related peptide and the above mentioned peptides were seen in close contact with the GR-IR neurons. These results suggest that the glucocorticoids may modulate directly the neurotransmitter synthesis of the CRF-, met-ENK, NT-, SOM- and SP-IR cells in the ACe.
中央杏仁核（ACe）是杏仁复合体的一部分，参与促肾上腺皮质激素分泌、应激相关反应及行为功能。ACe含有众多糖皮质激素受体（GR）免疫反应性（IR）神经元，此外，还已证实其含有几种神经肽IR胞体和神经末梢。为研究GR与神经肽IR结构之间的关系，我们绘制了杏仁复合体中GR样免疫反应性（LI）的分布图，并使ACe中的神经肽LI和GR-LI共定位。在杏仁复合体中，中央核、内侧核和皮质核含有大量GR-IR神经元，而在基底外侧核和基底内侧核中观察到中等数量的GR-IR神经元。在外侧核中仅见到少数GR-IR神经元。在ACe中，大多数促肾上腺皮质激素释放因子（CRF）、甲硫氨酸脑啡肽（met-ENK）、神经降压素（NT）和生长抑素（SOM）-IR神经元也含有GR-IR。约一半的P物质（SP）-IR神经元含有GR-IR，而少数血管活性肠肽和胆囊收缩素-IR神经元中只有一些显示出GR-LI。可见含有降钙素基因相关肽和上述肽的神经末梢与GR-IR神经元紧密接触。这些结果表明，糖皮质激素可能直接调节ACe中CRF、met-ENK、NT、SOM和SP-IR细胞的神经递质合成。
