从一只非洲绿猴身上分离出的猿猴免疫缺陷病毒SIVagmTYO - 7的内源性逆转录酶反应产物的特征分析。

Characterization of the endogenous reverse transcriptase reaction products of SIVagmTYO-7, a simian immunodeficiency virus isolated from an African green monkey.

作者信息

Lüke W, Dirks B, Moosmayer D, Jentsch K D, Hunsmann G

机构信息

Deutsches Primatenzentrum, Abteilung Virologie und Immunologie, Göttingen, Germany.

出版信息

Virus Res. 1992 Feb;22(2):143-9. doi: 10.1016/0168-1702(92)90040-g.

DOI:10.1016/0168-1702(92)90040-g

PMID:1373556

Abstract

The products of the endogenous reverse transcriptase reaction of SIVagmTYO-7 were characterized after the reaction conditions had been optimized. The major reaction product in the presence of actinomycin D and oligo(dT) was a DNA with a size of 300 bases. Without actinomycin D two additional reaction products with 600 or 700 bases appeared. The 300 base product was identified as the (-)strong-stop DNA, whereas the 700 base product is the (+)strong-stop DNA. The 600 base product appeared only after oligo(dT) priming. The (-)strong-stop DNA hybridized specifically with a 9 kb RNA found in virus particles and three RNA species of 1.8, 4.8 and 9 kb isolated from SIVagmTYO-7 infected cells.

摘要

在优化反应条件后，对SIVagmTYO - 7内源性逆转录酶反应的产物进行了表征。在放线菌素D和寡聚（dT）存在下的主要反应产物是大小为300个碱基的DNA。没有放线菌素D时，出现了另外两种大小为600或700个碱基的反应产物。300个碱基的产物被鉴定为（-）强终止DNA，而700个碱基的产物是（+）强终止DNA。600个碱基的产物仅在寡聚（dT）引发后出现。（-）强终止DNA与病毒颗粒中发现的9 kb RNA以及从感染SIVagmTYO - 7的细胞中分离出的1.8、4.8和9 kb的三种RNA特异性杂交。