糖蛋白加工抑制剂在肌发生的早期阶段起作用。

Inhibitors of glycoprotein processing act at an early stage of myogenesis.

作者信息

Trudel G C, Holland P C

机构信息

Muscle Biochemistry Laboratories, Montréal Neurological Institute McGill University, Que., Canada.

出版信息

Biochem Biophys Res Commun. 1992 Apr 15;184(1):125-30. doi: 10.1016/0006-291x(92)91167-o.

DOI:10.1016/0006-291x(92)91167-o

PMID:1373610

Abstract

The glycoprotein processing inhibitors bromoconduritol and N-methyl-1-deoxynojirimycin inhibit myoblast fusion and differentiation, suggesting the critical involvement of one or more glycoproteins in the control of skeletal myogenesis. In the present study we have examined the effect of inhibitors of glycoprotein processing on the expression of the muscle-specific regulatory factor myogenin. Glucosidase inhibitors, but not the mannosidase inhibitor 1-deoxymannojirimycin, inhibited the accumulation of myogenin mRNA in myoblasts, and immunoblotting confirmed that this was reflected in reduced accumulation of myogenin protein. The results indicate that the glycoprotein(s) critically involved in the control of myoblast differentiation act at an early stage in this process by modulating expression of the myogenic regulatory factor myogenin.

摘要

糖蛋白加工抑制剂溴代康杜醇和N-甲基-1-脱氧野尻霉素可抑制成肌细胞融合和分化，这表明一种或多种糖蛋白在骨骼肌生成的调控中起关键作用。在本研究中，我们检测了糖蛋白加工抑制剂对肌肉特异性调节因子肌细胞生成素表达的影响。葡糖苷酶抑制剂而非甘露糖苷酶抑制剂1-脱氧甘露野尻霉素可抑制成肌细胞中肌细胞生成素mRNA的积累，免疫印迹证实这反映在肌细胞生成素蛋白积累的减少上。结果表明，在成肌细胞分化调控中起关键作用的糖蛋白在此过程的早期阶段通过调节肌源性调节因子肌细胞生成素的表达发挥作用。