Reginato A J, Maldonado I, Reginato A M, Falasca G F, O'Connor C R
University of Medicine and Dentistry of New Jersy, Robert Wood Johnson Medical School, Camden.
Diagn Cytopathol. 1992;8(2):147-52. doi: 10.1002/dc.2840080211.
We explored the use of Testsimplet (TS) in synovial fluid (SF) analysis. TS is a glass slide coated with a dry mixture of methylene blue and cresyl violet, which in contact with one drop of SF provides a stained fresh preparation. We applied the TS to the study of 159 SFs of patients with different rheumatic diseases. In those SFs of patients with crystal-associated diseases, the crystal search was performed both on unstained preparations and with TS. TS was as good as the Wright's and Papanicolaou stain in characterizing SF cells, lupus erythematosus cells, and detection of occasional bacteria. TS allowed a better visualization of Reiter's cells, cartilage fragments, synovial villi, fat droplets, and fibrin. Crystals were identified in every TS of those patients with crystal-associated diseases. TS is a rapid and reproducible method of SF supravital staining. Crystals are well preserved for simultaneous examination with compensated polarized light.
我们探讨了Testsimplet（TS）在滑液（SF）分析中的应用。TS是一种涂有亚甲蓝和甲酚紫干混合物的载玻片，与一滴滑液接触后可提供染色的新鲜标本。我们将TS应用于159例不同风湿性疾病患者的滑液研究。在患有晶体相关疾病患者的那些滑液中，在未染色标本和使用TS的情况下都进行了晶体搜索。在表征滑液细胞、红斑狼疮细胞和检测偶尔出现的细菌方面，TS与瑞氏染色和巴氏染色效果相当。TS能更好地显示赖特细胞、软骨碎片、滑膜绒毛、脂肪滴和纤维蛋白。在患有晶体相关疾病的患者的每个TS标本中都鉴定出了晶体。TS是一种快速且可重复的滑液活体染色方法。晶体保存良好，可同时用补偿偏振光进行检查。
