Hudak S, Thompson-Snipes L, Rocco C, Jackson J, Pearce M, Rennick D
Department of Immunology, DNAX Research Institute, Palo Alto, California 94304.
Exp Hematol. 1992 May;20(4):412-7.
Interleukin 6 (IL-6) is one of several hemopoietic growth factors produced by stromal cell lines derived from the adherent layer of long-term bone marrow cultures (LTBMCs). To evaluate the potential role of IL-6 in stromal cell-dependent myelopoiesis, we established LTBMCs and verified that IL-6 mRNA is transcribed by heterogeneous adherent cell layers and that IL-6 protein is present in culture supernatants. Established LTBMCs were then depleted of IL-6 by using a specific neutralizing monoclonal antibody (mAb). Cultures treated for 2-3 weeks with anti-IL-6 mAb showed decreased production of maturing myeloid cells and colony-forming progenitor cells (colony-forming units in culture, CFU-c) but not stem cells (spleen colony-forming units, CFU-s). In parallel experiments, it was also found that the addition of IL-6 to LTBMCs stimulated a marked increase in total cell production, CFU-c, and day-8 CFU-s. In sum, it appears that endogenous production of IL-6, although limiting, is essential for the normal level of myelopoiesis associated with stromal cell function in LTBMCs.
白细胞介素6（IL-6）是由长期骨髓培养物（LTBMCs）贴壁层来源的基质细胞系产生的几种造血生长因子之一。为了评估IL-6在基质细胞依赖性骨髓生成中的潜在作用，我们建立了LTBMCs，并证实IL-6 mRNA由异质性贴壁细胞层转录，且IL-6蛋白存在于培养上清液中。然后，通过使用特异性中和单克隆抗体（mAb）去除已建立的LTBMCs中的IL-6。用抗IL-6 mAb处理2 - 3周的培养物显示，成熟髓细胞和集落形成祖细胞（培养中的集落形成单位，CFU-c）的产生减少，但干细胞（脾集落形成单位，CFU-s）未减少。在平行实验中，还发现向LTBMCs中添加IL-6可刺激总细胞产量、CFU-c和第8天CFU-s显著增加。总之，内源性IL-6的产生虽然有限，但对于LTBMCs中与基质细胞功能相关的正常骨髓生成水平至关重要。
