通过表位作图对人甲状旁腺激素N端抗体的特性进行鉴定。
Characterization of antibodies against human N-terminal parathyroid hormone by epitope mapping.
作者信息
Tampe J, Broszio P, Manneck H E, Missbichler A, Blind E, Müller K B, Schmidt-Gayk H, Armbruster F P
机构信息
Immundiagnostik, Bensheim, Germany.
出版信息
J Immunoassay. 1992;13(1):1-13. doi: 10.1080/15321819208019821.
Two polyclonal antisera from goat and mouse and two monoclonal antibodies against human parathyroid hormone (1-34) were characterised by epitope mapping. Hexapeptides were synthesized on polystyrene pins, the sequences of which overlapped and represented the entire sequence of hPTH(1-34). Binding of antibodies to these hexapeptides was determined and antigenic determinants thus characterized. At least one predominant binding sequence was detected in the region of hPTH(7-14).
通过表位作图对来自山羊和小鼠的两种多克隆抗血清以及两种抗人甲状旁腺激素（1-34）的单克隆抗体进行了表征。在聚苯乙烯针上合成六肽，其序列相互重叠并代表hPTH(1-34)的完整序列。测定抗体与这些六肽的结合情况，从而表征抗原决定簇。在hPTH(7-14)区域检测到至少一个主要结合序列。
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