Kojima H, Konishi H, Kuroda Y
Biochemical Research Institute, Nippon Menard Cosmetic Co. Ltd., Gifu, Japan.
Mutat Res. 1992 Apr;266(2):171-80. doi: 10.1016/0027-5107(92)90184-4.
The combined effects of methyl methanesulfonate (MMS) and ethyl methanesulfonate (EMS) on the induction of 6-thioguanine (6TG)-resistant mutants and chromosome aberrations were examined in Chinese hamster V79 cells. Cells were simultaneously treated with EMS at a concentration of D20 and MMS at various concentrations for 3, 6 or 9 h. In other experiments cells were simultaneously treated with MMS at a concentration of D20 and EMS at various concentrations for 3, 6 or 9 h. The mathematical analysis of the combined effects of both chemicals for cell killing (cytotoxicity) and 6TG-resistant mutations indicates that synergistic interactions were observed for both cell killing and mutations induced by MMS and EMS. The frequency of chromosome aberrations induced by simultaneous treatment with MMS at a concentration of D20 and EMS at various concentrations for 3 h was additive. However, the frequency of chromosome aberrations induced by EMS at a concentration of D20 and MMS at various concentrations for 3 h was not significantly different from those induced by MMS alone.
研究了甲磺酸甲酯（MMS）和乙磺酸乙酯（EMS）联合作用对中国仓鼠V79细胞中6-硫鸟嘌呤（6TG）抗性突变体诱导及染色体畸变的影响。细胞同时用浓度为D20的EMS和不同浓度的MMS处理3、6或9小时。在其他实验中，细胞同时用浓度为D20的MMS和不同浓度的EMS处理3、6或9小时。对两种化学物质联合作用于细胞杀伤（细胞毒性）和6TG抗性突变的数学分析表明，MMS和EMS诱导的细胞杀伤和突变均观察到协同相互作用。浓度为D20的MMS与不同浓度的EMS同时处理3小时诱导的染色体畸变频率呈相加性。然而，浓度为D20的EMS与不同浓度的MMS同时处理3小时诱导的染色体畸变频率与单独用MMS诱导的频率无显著差异。
