Belouchi A, Bradley W E
Institut du Cancer de Montréal, Que., Canada.
Mutat Res. 1992 Apr;266(2):221-30. doi: 10.1016/0027-5107(92)90190-d.
Early work with adenine phosphoribosyltransferase-deficient mutants of CHO cells suggested that a site existed in the third exon of this gene which was preferentially susceptible to mutation by ethyl methanesulphonate. To determine whether this was real we analysed a large collection of induced mutants, and generated a high-density mutational spectrum for this exon. In addition, 4 sites outside exon 3 were analysed by blot. 37 mutations were found in 19 available sites, six of which were at nucleotide 1365, 1 of 2 sites in the putative hotspot (P less than 0.02). One other site, 1308, also was mutated in 6 cell lines and may also be preferentially mutable.
早期对CHO细胞腺嘌呤磷酸核糖转移酶缺陷型突变体的研究表明，该基因的第三个外显子中存在一个位点，该位点对甲磺酸乙酯诱导的突变特别敏感。为了确定这是否属实，我们分析了大量诱导突变体，并为该外显子生成了高密度突变谱。此外，通过印迹分析了外显子3以外的4个位点。在19个可用位点中发现了37个突变，其中6个位于核苷酸1365处，在假定的热点区域的2个位点中有1个发生突变（P<0.02）。另一个位点1308在6个细胞系中也发生了突变，也可能是易突变位点。
