非甲非乙型肝炎患者丙型肝炎病毒标志物的比较

Comparison of hepatitis C virus markers in patients with NANB hepatitis.

作者信息

Yatsuhashi H, Inoue O, Koga M, Nagataki S, Mizuno K, Kolberg J, Beall E, Cha T A, Irvine B, Kuo G

机构信息

Department of Clinical Research, Nagasaki Chuo National Hospital, Japan.

出版信息

J Virol Methods. 1992 Apr;37(1):13-21. doi: 10.1016/0166-0934(92)90017-8.

DOI:10.1016/0166-0934(92)90017-8

PMID:1374072

Abstract

10 different HCV-specific assays and RT-PCR of the 5' untranslated region of HCV RNA were used to analyze sixty-four patients with chronic NANB liver disease. Po, CP-9 and C22 antigens are located in the putative core; C33c in the putative NS3; C100-3 in the putative NS3/4; KCL in the putative NS4/5 and C825 is located in the putative NS5. GOR protein is not part of the HCV genome, but antibodies to it appear to be present in response to a hepatitis C infection. Positive rates were 91% for Po, 89% for CP-9, 94% for C22, 97% for C33c, 88% for C100-3 (Ortho, EIA), 86% for C100-3 (Abbott, EIA), 84% for C100-3 (Ohtsuka, RIA), 88% for KCL, 59% for C825, 58% for GOR, and 83% for RT-PCR. There were 8 cases which were negative by all anti-C100 tests. 7 of these cases were positive by other anti-HCV markers and/or PCR suggesting the need for improved blood screening assays. There is a variation in the relative reactivity for different markers with different samples. Of the tests employed, anti C33c shows the highest positivity rate.

摘要

使用10种不同的丙型肝炎病毒（HCV）特异性检测方法以及HCV RNA 5'非翻译区的逆转录聚合酶链反应（RT-PCR）对64例慢性非甲非乙型肝病患者进行分析。Po、CP-9和C22抗原位于假定的核心区域；C33c位于假定的NS3区域；C100-3位于假定的NS3/4区域；KCL位于假定的NS4/5区域，C825位于假定的NS5区域。GOR蛋白不是HCV基因组的一部分，但针对它的抗体似乎在丙型肝炎感染后出现。Po的阳性率为91%，CP-9为89%，C22为94%，C33c为97%，C100-3（Ortho，酶免疫测定）为88%，C100-3（雅培，酶免疫测定）为86%，C100-3（大冢，放射免疫测定）为84%，KCL为88%，C825为59%，GOR为58%，RT-PCR为83%。有8例患者所有抗C100检测均为阴性。其中7例通过其他抗HCV标志物和/或PCR检测呈阳性，这表明需要改进血液筛查检测方法。不同标志物对不同样本的相对反应性存在差异。在所采用的检测中，抗C33c显示出最高的阳性率。