Nagata S, Ishii H, Yokoyama H, Kato S, Moriya S, Maruyama K, Takahashi H, Tsuchiya M
Department of Internal Medicine, Kitasato Institute Medical Center Hospital, Saitama, Japan.
Gastroenterol Jpn. 1993 May;28 Suppl 5:91-4. doi: 10.1007/BF02989215.
Since the assay of HCV antibody has been developed, it became clear that HCV is involved not only in patients with non-A non-B hepatitis but also in some alcoholic patients. The aim of this study is to examine the prevalence of HCV in chronic alcoholics and to elucidate the influence of HCV and its subtypes on pathogenesis and clinical feature of alcoholic liver disease. To that effect, sera from 100 alcoholics were tested for antibody against C100 of HCV by ELISA and for HCV RNA by reverse transcriptation and the PCR method. The incidence of HCV Ab/RNA was 0%/17% in patients with non specific reactive hepatitis, 0%/0% in fatty liver, 17%/17% in alcoholic hepatitis, 50%/73% in chronic hepatitis, 15%/18% in liver fibrosis and 19%/31% in liver cirrhosis. HCV Ab and RNA were positive in 21% and 31% of alcoholics, respectively. Subtypes of HCV were identified by dot blot hybridization method. Type K1, K2a and K2b were detected in 68%, 25% and 7% of 28 patients with positive HCV RNA, respectively. Type PT was not detected. In addition, serum transaminase activities were evaluated after 4 weeks abstinence. The incidence of normalization of the enzyme activity was lower in patients with positive HCV RNA than that with negative HCV RNA. Furthermore, when it was estimated in relation to HCV subtypes, the incidence of normalization in patients with type K1 was lower than that with type K2. In conclusion, the prevalence of HCV infection in alcoholic patients was much greater than general population.(ABSTRACT TRUNCATED AT 250 WORDS)
自从丙型肝炎病毒（HCV）抗体检测方法问世以来，人们清楚地认识到HCV不仅与非甲非乙型肝炎患者有关，还与一些酗酒患者有关。本研究的目的是检测慢性酗酒者中HCV的流行情况，并阐明HCV及其亚型对酒精性肝病发病机制和临床特征的影响。为此，采用酶联免疫吸附测定（ELISA）法检测了100例酗酒者血清中抗HCV C100抗体，并采用逆转录聚合酶链反应（RT-PCR）法检测HCV RNA。非特异性反应性肝炎患者中HCV抗体/RNA的发生率为0%/17%，脂肪肝患者为0%/0%，酒精性肝炎患者为17%/17%，慢性肝炎患者为50%/73%，肝纤维化患者为15%/18%，肝硬化患者为19%/31%。酗酒者中HCV抗体和RNA阳性率分别为21%和31%。采用斑点杂交法鉴定HCV亚型。在28例HCV RNA阳性患者中，分别检测到K1型、K2a型和K2b型，比例分别为68%、25%和7%。未检测到PT型。此外，在戒酒4周后评估血清转氨酶活性。HCV RNA阳性患者酶活性恢复正常的发生率低于HCV RNA阴性患者。此外，按HCV亚型评估时，K1型患者酶活性恢复正常的发生率低于K2型患者。总之，酗酒患者中HCV感染的流行率远高于普通人群。（摘要截短于250字）
