Alifano P, Piscitelli C, Blasi V, Rivellini F, Nappo A G, Bruni C B, Carlomagno M S
Dipartimento di Biologia e Patologia Cellulare e Molecolare, Centro di Endocrinologia ed Oncologia Sperimentale del Consiglio Nazionale delle Richerche, Università di Napoli, Italy.
Mol Microbiol. 1992 Mar;6(6):787-98. doi: 10.1111/j.1365-2958.1992.tb01529.x.
We have characterized a major processed species of mRNA in the his operon of Salmonella typhimurium. In vivo and in vitro analyses of the his transcripts from wild-type and mutant strains using S1 nuclease protection assays, measurements of RNA stability, deletion mapping, gel retardation, and in vitro translation assays demonstrate that the distal portion of the polycistronic his mRNA is processed, resulting in increased stability. The processing event requires an upstream cis-acting element and translation of the cistron immediately downstream of the 5' end of the processed species. The cistrons contained in this segment are also independently transcribed from an internal promoter which is maximally active in the absence of readthrough transcription from the primary promoter.
我们已对鼠伤寒沙门氏菌组氨酸操纵子中一种主要的加工后mRNA种类进行了表征。利用S1核酸酶保护分析、RNA稳定性测量、缺失定位、凝胶阻滞和体外翻译分析，对野生型和突变株的组氨酸转录本进行体内和体外分析，结果表明，多顺反子组氨酸mRNA的远端部分被加工，从而提高了稳定性。加工事件需要一个上游顺式作用元件以及加工后物种5'端紧邻的顺反子的翻译。该片段中包含的顺反子也从一个内部启动子独立转录，该启动子在没有来自初级启动子的通读转录时活性最高。
