Lundy F T, Wisdom G B
Division of Biochemistry, Queen's University, Belfast, Northern Ireland, UK.
Clin Chim Acta. 1992 Feb 14;205(3):187-95. doi: 10.1016/0009-8981(92)90059-y.
Serum proteins were fractionated by polyacrylamide gel electrophoresis under denaturing conditions and transferred to nitrocellulose membranes. The blotted polypeptides were probed with biotinylated Ricinus communis lectin (RCA120) followed by streptavidin/alkaline phosphatase. This procedure detected five asialoglycoproteins (alpha 2-macroglobulin, transferrin, alpha 1-antitrypsin, alpha 1-antichymotrypsin and haptoglobin beta chain). The asialoform of the alpha 1-trypsin inhibitor was found to be decreased in inflammation.
在变性条件下通过聚丙烯酰胺凝胶电泳对血清蛋白进行分离，并转移至硝酸纤维素膜上。将印迹的多肽与生物素化的蓖麻凝集素（RCA120）进行杂交，随后与链霉亲和素/碱性磷酸酶反应。该方法检测到五种去唾液酸糖蛋白（α2-巨球蛋白、转铁蛋白、α1-抗胰蛋白酶、α1-抗糜蛋白酶和触珠蛋白β链）。发现α1-胰蛋白酶抑制剂的去唾液酸化形式在炎症中减少。
