Böhm D, Nick S, Voss G, Hunsmann G
German Primate Centre, Göttingen.
Cytometry. 1992;13(3):259-66. doi: 10.1002/cyto.990130307.
The human monocytic cell line U-937 clone 2 and two T-cell lines CEM and MOLT-4 clone 8 were infected with HIV-2ben, a recent isolate of HIV-2. Infection and subsequent antigen expression on the cell surface was monitored by flow cytometry using a rabbit-anti-serum against tween-ether-treated HIV-2ben and a fluorescein-isothiocyanate-conjugated IgG against rabbit-IgG. The sensitivity of the three cell lines to infection with HIV-2ben correlated with the percentages of CD4-expressing cells but not with the levels of CD4-expression on the cell. The appearance of viral surface antigens preceded the formation of syncytia and correlated closely with the infecting virus dose. After 1-2 weeks in culture, 20-85% of the cells of each line expressed viral surface antigens. The variation depended on the cell type and cell culture conditions. The MOLT-4 clone 8 and the U-937 clone 2 cells died around 10 or 20 days, respectively, after HIV-2ben infection. Only HIV-2ben infected CEM cells grew permanently. Flow cytometry was an appropriate method to monitor the expression of viral proteins on the cell surface of HIV-infected cell lines. Flow cytometry proved to be more sensitive than determination of RT activity in supernatants of HIV-infected cells and more precise than light microscopy examinations.
人单核细胞系U - 937克隆2以及两个T细胞系CEM和MOLT - 4克隆8被HIV - 2ben感染，HIV - 2ben是HIV - 2的一个近期分离株。使用针对吐温 - 乙醚处理的HIV - 2ben的兔抗血清以及针对兔IgG的异硫氰酸荧光素偶联IgG，通过流式细胞术监测细胞表面的感染及随后的抗原表达。这三种细胞系对HIV - 2ben感染的敏感性与表达CD4的细胞百分比相关，但与细胞上CD4的表达水平无关。病毒表面抗原的出现先于多核巨细胞的形成，并且与感染病毒剂量密切相关。培养1 - 2周后，每个细胞系中20 - 85%的细胞表达病毒表面抗原。这种差异取决于细胞类型和细胞培养条件。MOLT - 4克隆8细胞和U - 937克隆2细胞在被HIV - 2ben感染后分别在大约10天或20天死亡。只有被HIV - 2ben感染的CEM细胞能永久生长。流式细胞术是监测HIV感染细胞系细胞表面病毒蛋白表达的合适方法。流式细胞术被证明比测定HIV感染细胞上清液中的RT活性更敏感，并且比光学显微镜检查更精确。
