两种大鼠品系脑区中剪接变异的D2受体mRNA相对丰度的年龄相关变化。

Age-related variations in relative abundance of alternative spliced D2 receptor mRNAs in brain areas of two rat strains.

作者信息

Della Vedova F, Fumagalli F, Sacchetti G, Racagni G, Brunello N

机构信息

Center of Neuropharmacology, University of Milan, Italy.

出版信息

Brain Res Mol Brain Res. 1992 Feb;12(4):357-9. doi: 10.1016/0169-328x(92)90140-7.

DOI:10.1016/0169-328x(92)90140-7

PMID:1374501

Abstract

Age-related reduction in the steady-state levels of messenger RNA for D2(415) and D2(444), the alternatively spliced form of dopamine D2 receptors, was observed in different rat brain areas using the sensitive reverse transcription (RT)-polymerase chain reaction (PCR) technique. In both Sprague-Dawley and Wistar aged rats, the decrease was more pronounced in the D2(444) isoform mRNA thus leading to a changed ratio in striatum as well as in the hippocampus.

摘要

使用灵敏的逆转录（RT）-聚合酶链反应（PCR）技术，在不同的大鼠脑区观察到，随着年龄增长，多巴胺D2受体可变剪接形式D2(415)和D2(444)的信使核糖核酸稳态水平降低。在斯普拉格-道利大鼠和Wistar老龄大鼠中，D2(444)亚型信使核糖核酸的减少更为明显，从而导致纹状体以及海马体中的比例发生变化。