Elferink M G, de Wit J G, In 't Veld G, Reichert A, Driessen A J, Ringsdorf H, Konings W N
Department of Microbiology, University of Groningen, Haren, Netherlands.
Biochim Biophys Acta. 1992 Apr 29;1106(1):23-30. doi: 10.1016/0005-2736(92)90217-a.
Liposomes composed of Escherichia coli phospholipid were coated with polysaccharides bearing hydrophobic palmitoyl anchors. The effect on the stability of liposomes without or with integral membrane proteins was investigated. A high concentration of hydrophobized dextrans protected the liposomes against detergent degradation, decreased the fluidity of the membranes, prevented fusion of the liposomes and enhanced their stability. Proteoliposomes containing beef heart cytochrome-c oxidase and the lactose transport carrier of E. coli were similarly affected by coating with the dextrans. Under these conditions both membrane proteins were still active. Long-term stability of the coated liposomes was obtained only in the absence of the integral membrane proteins.
由大肠杆菌磷脂组成的脂质体用带有疏水棕榈酰锚的多糖包被。研究了对不含或含有整合膜蛋白的脂质体稳定性的影响。高浓度的疏水化葡聚糖可保护脂质体免受去污剂降解，降低膜的流动性，防止脂质体融合并增强其稳定性。含有牛心细胞色素c氧化酶和大肠杆菌乳糖转运载体的蛋白脂质体同样受到葡聚糖包被的影响。在这些条件下，两种膜蛋白仍然具有活性。仅在没有整合膜蛋白的情况下，包被的脂质体才能获得长期稳定性。
