Yu Q, Banerjee S D, Toole B P
Department of Anatomy and Cellular Biology, Tufts University Health Science Schools, Boston, Massachusetts 02111.
Dev Dyn. 1992 Feb;193(2):145-51. doi: 10.1002/aja.1001930206.
Hyaluronan-dependent pericellular matrices or "coats" are expressed by a variety of cell types in culture and modulation of their expression may be important in regulation of cell interactions in vivo during development. Monoclonal antibody IVd4, which recognizes hyaluronan-binding protein with the properties of a hyaluronan receptor, was shown to block formation of these coats by a variety of cells. Using rat fibrosarcoma cells, it was found that the antibody not only blocked initial formation of the coats but also caused their loss when added subsequent to formation. The loss of preformed coats in the presence of antibody occurred at 4 degrees and 37 degrees, implying that the function of hyaluronan-binding protein in coat formation is not in mediating metabolic processes. The antibody also had no significant effect on hyaluronan production by the fibrosarcoma cells. In addition, hyaluronan hexasaccharide, a competitive inhibitor of the interaction between polymeric hyaluronan and its cell surface receptor, was found to inhibit coat formation. Thus it is concluded that a hyaluronan-binding protein with the properties of a hyaluronan receptor is required for pericellular matrix formation.
透明质酸依赖性细胞周围基质或“被膜”在培养的多种细胞类型中表达，其表达的调节对于发育过程中体内细胞相互作用的调控可能很重要。单克隆抗体IVd4可识别具有透明质酸受体特性的透明质酸结合蛋白，已证明它能阻止多种细胞形成这些被膜。利用大鼠纤维肉瘤细胞发现，该抗体不仅能阻止被膜的初始形成，而且在被膜形成后添加时还会导致其消失。在抗体存在的情况下，预先形成的被膜在4℃和37℃时都会消失，这意味着透明质酸结合蛋白在被膜形成中的功能并非介导代谢过程。该抗体对纤维肉瘤细胞产生透明质酸也没有显著影响。此外，还发现透明质酸六糖，一种聚合透明质酸与其细胞表面受体相互作用的竞争性抑制剂，可抑制被膜形成。因此得出结论，细胞周围基质形成需要具有透明质酸受体特性的透明质酸结合蛋白。
