Detection of a CpA methylase in an insect system: characterization and substrate specificity.
作者信息
Devajyothi C, Brahmachari V
机构信息
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore.
出版信息
Mol Cell Biochem. 1992 Mar 25;110(2):103-11. doi: 10.1007/BF02454187.
PMID:1374836
Abstract
A cytosine-specific DNA methyltransferase (EC 2.1.1.37) has been purified to near homogeneity from a mealybug (Planococcus lilacinus). The enzyme can methylate cytosine residues in CpG sequences as well as CpA sequences. The apparent molecular weight of the enzyme was estimated as 135,000 daltons by FPLC. The enzyme exhibits a processive mode of action and a salt dependence similar to mammalian methylases. Mealybug methylase exhibits a preference for denatured DNA substrates.
摘要
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