单克隆抗体PCG-4识别的艰难梭菌毒素A上两个表位的定位
Localization of two epitopes recognized by monoclonal antibody PCG-4 on Clostridium difficile toxin A.
作者信息
Frey S M, Wilkins T D
机构信息
Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061.
出版信息
Infect Immun. 1992 Jun;60(6):2488-92. doi: 10.1128/iai.60.6.2488-2492.1992.
Abstract
The toxin A gene of Clostridium difficile contains a 2.5-kb region encoding a series of contiguous repeating units located at the COOH terminus of the molecule. We previously showed that the monoclonal antibody (MAb) PCG-4, which neutralizes the enterotoxic activity of toxin A, binds to epitopes located within these repeating units. In the present study, we subcloned a series of fragments from this portion of the gene. The recombinant peptides expressed from the gene fragments were examined for reactivity with MAb PCG-4 to identify the epitopes involved in binding. Our results showed that MAb PCG-4 recognizes epitopes in amino acid residues 2097 through 2141 and amino acid residues 2355 through 2398.
摘要
艰难梭菌的毒素A基因包含一个2.5 kb的区域，该区域编码位于分子COOH末端的一系列连续重复单元。我们之前表明，中和毒素A肠毒素活性的单克隆抗体（MAb）PCG-4与这些重复单元内的表位结合。在本研究中，我们从该基因的这一部分亚克隆了一系列片段。检测从基因片段表达的重组肽与MAb PCG-4的反应性，以鉴定参与结合的表位。我们的结果表明，MAb PCG-4识别2097至2141位氨基酸残基以及2355至2398位氨基酸残基中的表位。
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