血管活性肠肽调节大鼠视网膜双极细胞和神经节细胞中GABAA受体的功能。

Vasoactive intestinal polypeptide modulates GABAA receptor function in bipolar cells and ganglion cells of the rat retina.

Veruki M L, Yeh H H

Program in Neuroscience, University of Rochester School of Medicine and Dentistry, New York 14642.

J Neurophysiol. 1992 Apr;67(4):791-7. doi: 10.1152/jn.1992.67.4.791.

Abstract

The effect of vasoactive intestinal polypeptide (VIP) on bipolar cells and ganglion cells freshly dissociated from the rat retina was studied under voltage clamp with the use of patch-clamp recording in the whole-cell configuration. 2. Application of VIP (1-100 microM) by itself resulted in no detectable current response in either bipolar cells or ganglion cells. However, gamma-aminobutyric acid (GABA)-activated macroscopic current responses elicited in both neuronal populations were potentiated on superimposed exposure to the neuropeptide. 3. GABA-activated chloride currents and muscimol-induced current responses were similarly potentiated on exposure to VIP, suggesting a synergistic interaction between VIP and GABAA receptor mechanisms. 4. We postulate that VIP plays a neuromodulatory role by regulating the excitability of inner retinal neurons and in this way modulates the efficacy of synaptic transmission in the retina.

摘要

采用全细胞膜片钳记录技术，在电压钳条件下研究了血管活性肠肽（VIP）对新鲜分离的大鼠视网膜双极细胞和神经节细胞的作用。2. 单独应用VIP（1 - 100微摩尔）时，双极细胞和神经节细胞均未检测到电流反应。然而，在这两种神经元群体中，γ-氨基丁酸（GABA）激活的宏观电流反应在叠加暴露于该神经肽时增强。3. 暴露于VIP时，GABA激活的氯离子电流和蝇蕈醇诱导的电流反应同样增强，提示VIP与GABAA受体机制之间存在协同相互作用。4. 我们推测，VIP通过调节视网膜内层神经元的兴奋性发挥神经调节作用，进而调节视网膜中突触传递的效能。