Sachs D H, Leight G, Cone J, Schwarz S, Stuart L, Rosenberg S
Transplantation. 1976 Dec;22(6):559-67. doi: 10.1097/00007890-197612000-00004.
Three strains of miniature swine, each homozygous for a different allele of the major histocompatibility locus (MHC), have been developed by a selective breeding scheme based on tissue typing of the offspring of each generation. Prior to breeding, the original parents were reciprocally immunized by skin grafts and lymphocyte injections to produce lymphocytotoxic antisera. These antisera were then used to assess the MHC genotype of the offspring by an analysis based on selective absorption of cytotoxicity. Offspring inheriting the same serologically determined genotype were then bred sequentially. Subsequent mixed lymphocyte cultures showed a pattern of reactivity consistent with the serological genotyping, further confirming the homology between the MHC of these miniature swine and those of man and mouse. In addition to their usefulness as a model for large animal surgical transplantation, these animals provide an abundant homozygous source of histocompatibility antigens and of antihistocompatibility antisera for use in chemical characterization of products of the MHC.
通过基于每代后代组织分型的选择性育种方案，已培育出三种微型猪品系，每种品系在主要组织相容性位点（MHC）上对于不同的等位基因都是纯合的。在育种之前，原始亲本通过皮肤移植和淋巴细胞注射进行相互免疫，以产生淋巴细胞毒性抗血清。然后，通过基于细胞毒性选择性吸收的分析，使用这些抗血清来评估后代的MHC基因型。然后依次培育继承相同血清学确定基因型的后代。随后的混合淋巴细胞培养显示出与血清学分型一致的反应模式，进一步证实了这些微型猪的MHC与人及小鼠的MHC之间的同源性。除了作为大型动物外科移植模型有用之外，这些动物还提供了丰富的组织相容性抗原纯合来源以及抗组织相容性抗血清，用于MHC产物的化学表征。
