Solary E, Casasnovas R O, Campos L, Béné M C, Faure G, Maingon P, Falkenrodt A, Lenormand B, Genetet N
Unité d'Hématologie Clinique, C.H.U. Le Bocage, Dijon, France.
Leukemia. 1992 May;6(5):393-9.
The immunophenotype of blast cells was investigated in a multicentric study of 154 adult acute myeloblastic leukemias (AMLs). A panel of 27 monoclonal antibodies (MoAbs) was tested in indirect immunofluorescence. Expression of CD14 (UCHM1), CD19 (SB4), CD36 (OKM5), and HLA-DR were associated with higher mean leucocyte counts. CD14 expression correlated with low hemoglobin level and the absence of CD33 (MY9) with low platelet counts. Extramedullary disease was associated with CD16 (Leu11b) and HLA-DR antigen positivity. The study of relationships between surface markers and FAB criteria confirmed the predominant expression of CD14 in the M5 sub-group (p less than 0.000001) and the association of CD19 and CD36 with monocytic M4/M5 subgroups (respectively p less than 0.00002 and p less than 0.000001). All patients received an induction therapy including an anthracycline and cytarabine. The median follow-up was 13 months. The achievement of complete remission (CR) was inversely correlated with CD34 (B13C5) and CD19 expression: CR was obtained in 31 of 59 (53%) CD34-positive AML versus 64 of 75 (85%) CD34-negative cases (p less than 0.0001) and 11 of 24 (46%) CD19-positive versus 95 of 122 (78%) CD19-negative cases (p less than 0.01). In univariate analysis, a longer survival was associated with CD33 expression and the combined phenotypes CD36+/CD19- and CD16+/CD14-. Conversely, the CD18(IOT18)+/CDw65(VIM2)- phenotype was related to shorter survival. The expression of CD19, of CD34, and of the combined phenotype CD14+/DR--correlated with shorter survival as demonstrated both in univariate and multivariate analysis (p less than 0.03 in each case in multivariate analysis).
在一项对154例成人急性髓系白血病（AML）的多中心研究中，对原始细胞的免疫表型进行了调查。使用一组27种单克隆抗体（MoAb）进行间接免疫荧光检测。CD14（UCHM1）、CD19（SB4）、CD36（OKM5）和HLA-DR的表达与较高的平均白细胞计数相关。CD14表达与低血红蛋白水平相关，而CD33（MY9）缺失与低血小板计数相关。髓外疾病与CD16（Leu11b）和HLA-DR抗原阳性相关。表面标志物与FAB标准之间关系的研究证实，CD14在M5亚组中主要表达（p小于0.000001），CD19和CD36与单核细胞M4/M5亚组相关（分别为p小于0.00002和p小于0.000001）。所有患者均接受了包括蒽环类药物和阿糖胞苷的诱导治疗。中位随访时间为13个月。完全缓解（CR）的实现与CD34（B13C5）和CD19表达呈负相关：59例CD34阳性AML中有31例（53%）达到CR，而75例CD34阴性病例中有64例（85%）达到CR（p小于0.0001）；24例CD19阳性病例中有11例（46%）达到CR，而122例CD19阴性病例中有95例（78%）达到CR（p小于0.01）。在单变量分析中，较长的生存期与CD33表达以及CD36+/CD19-和CD16+/CD14-联合表型相关。相反，CD18（IOT18）+/CDw65（VIM2）-表型与较短的生存期相关。单变量和多变量分析均显示，CD19、CD34以及CD14+/DR-联合表型的表达与较短的生存期相关（多变量分析中每种情况p均小于0.03）。
