Shanazarova I M, Vanchugova L V, Valuev L I, Platé N A
Prikl Biokhim Mikrobiol. 1992 Mar-Apr;28(2):292-6.
The interaction of ovomucoid proteinase inhibitor prepared from duck egg white with a dextran of a molecular weight of 70,000 preliminary treated with potassium periodate. Irrespective of the number of the sites of the ovomucoid binding to aldehyde-dextran the anti-chymotryptic activity is equal to that of the native inhibitor, while the antitryptic activity decreases proportionally to the number of ovomucoid amino groups involved in the reaction with dextran. When a few ovomucoid molecules are immobilized on the polysaccharide macromolecule the perturbing effect of the protein-protein interactions is minimal, as the rigid polymeric chain prevents from the formation of associates of proteins immobilized on this backbone.
用高碘酸钾预处理的分子量为70000的葡聚糖与从鸭蛋清中制备的卵类粘蛋白蛋白酶抑制剂之间的相互作用。无论卵类粘蛋白与醛基葡聚糖结合位点的数量如何，抗胰凝乳蛋白酶活性都与天然抑制剂的活性相当，而抗胰蛋白酶活性则与参与葡聚糖反应的卵类粘蛋白氨基数量成比例降低。当一些卵类粘蛋白分子固定在多糖大分子上时，蛋白质-蛋白质相互作用的干扰作用最小，因为刚性聚合物链可防止固定在该主链上的蛋白质形成缔合体。
