Torsello A, Vrontakis M E, Schroedter I C, Vuille J C, Ikejiani C, Friesen H G
Department of Physiology, University of Manitoba, Winnipeg, Canada.
Endocrinology. 1992 Jun;130(6):3301-6. doi: 10.1210/endo.130.6.1375899.
Galanin is a neuropeptide widely distributed throughout the vertebrate neural and endocrine system. Galanin can influence pituitary hormone secretion, intestinal motility, and other biological activities. The precise physiological role of galanin is unknown. We studied the control of galanin gene expression in peripheral organs in the male rat using Northern blot and in situ hybridization techniques. In the adrenals and prostate, galanin mRNA was undetectable in the controls and did not change after the administration of dexamethasone (0.0001-10.0 mg/kg, ip) and diethylstilbestrol (0.1 mg/kg, ip). In the testis, thymus, seminal vesicles, medial basal hypothalamus, and colon, galanin message was detectable, but was not influenced by steroids. On the other hand, dexamethasone (0.5-10.0 mg/kg) was very effective in enhancing galanin expression in the vas deferens and epididymis (4- to 7-fold in the vas deferens), with a peak 6-9 h after the treatment. Diethylstilbestrol (0.1 mg/kg) stimulated galanin mRNA transcription only in the vas deferens (2- to 3-fold), with a peak 1-3 h after the treatment. Dihydrotestosterone treatment (0.2-0.4 mg/kg) was ineffective in all tissues examined. In the vas deferens and seminal vesicles, galanin mRNA has been localized at a cellular level by in situ hybridization. In these tissues only fibroblast-like cells contained the message. These data demonstrate that galanin is expressed in the male rat reproductive system and that steroid hormones participate in the control of galanin gene expression in a tissue- and hormone-specific fashion.
甘丙肽是一种广泛分布于整个脊椎动物神经和内分泌系统的神经肽。甘丙肽可影响垂体激素分泌、肠道蠕动及其他生物活性。甘丙肽的确切生理作用尚不清楚。我们使用Northern印迹法和原位杂交技术研究了雄性大鼠外周器官中甘丙肽基因表达的调控。在肾上腺和前列腺中，对照组未检测到甘丙肽mRNA，给予地塞米松（0.0001 - 10.0 mg/kg，腹腔注射）和己烯雌酚（0.1 mg/kg，腹腔注射）后也未发生变化。在睾丸、胸腺、精囊、内侧基底下丘脑和结肠中，可检测到甘丙肽信息，但不受类固醇影响。另一方面，地塞米松（0.5 - 10.0 mg/kg）能非常有效地增强输精管和附睾中甘丙肽的表达（输精管中增加4至7倍），给药后6至9小时达到峰值。己烯雌酚（0.1 mg/kg）仅刺激输精管中甘丙肽mRNA转录（增加2至3倍），给药后1至3小时达到峰值。双氢睾酮处理（0.2 - 0.4 mg/kg）在所有检测组织中均无效。通过原位杂交在细胞水平上对输精管和精囊中甘丙肽mRNA进行了定位。在这些组织中，只有成纤维细胞样细胞含有该信息。这些数据表明，甘丙肽在雄性大鼠生殖系统中表达，类固醇激素以组织和激素特异性方式参与甘丙肽基因表达的调控。
