[利用凝集素研究软体动物神经元中不同亚型胆碱能受体的碳水化合物决定簇]
[Use of lectins for the study of carbohydrate determinants of cholinoreceptors of various subtypes in mollusk neurons].
作者信息
Diatlov V A
出版信息
Neirofiziologiia. 1992;24(2):161-9.
Chemical nature of carbohydrate components in identified neurons of mollusc Helix pomatia influencing acetylcholine responses was investigated using different lectins (Con A, RCA, WGA and LPA). Differences between Con A-evoked changes in acetylcholine-induced chloride and sodium-potassium currents as well as differences in the time and temperature dependences of these changes showed various mechanisms of action of carbohydrate component with mannose residues on the function of acetylcholine receptors. The analysis of the time and temperature dependences of depressing effects of WGA and RCA on acetylcholine-induced currents permits supposing endocytosis of lectin-receptor complexes with N-acetyl-D-glucosamine residues.
利用不同的凝集素（伴刀豆球蛋白A、蓖麻凝集素、小麦胚凝集素和扁豆凝集素），研究了影响乙酰胆碱反应的苹果螺特定神经元中碳水化合物成分的化学性质。伴刀豆球蛋白A诱发的乙酰胆碱诱导的氯离子和钠钾电流变化之间的差异，以及这些变化在时间和温度依赖性上的差异，表明具有甘露糖残基的碳水化合物成分对乙酰胆碱受体功能有多种作用机制。对小麦胚凝集素和蓖麻凝集素对乙酰胆碱诱导电流的抑制作用的时间和温度依赖性分析，推测含有N-乙酰-D-葡萄糖胺残基的凝集素-受体复合物会发生内吞作用。
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