LEVENE C I
J Exp Med. 1961 Apr 1;113(4):795-810. doi: 10.1084/jem.113.4.795.
When applied to the chorio-allantoic membrane of the chick embryo, isoniazid was shown to produce an increase in the fragility of the embryo and in the amount of collagen which was extractable from the bones with cold 1 M sodium chloride. The administration of pyridoxal reversed these phenomena almost completely. The effect of isoniazid differed from that of beta-aminopropionitrile in that the latter was of greater magnitude, and was not affected by pyridoxal; whereas beta-aminopropionitrile caused skeletal deformities, isoniazid even at 12 times the concentration produced no deformities. The aldehyde group of pyridoxal was shown to be necessary for its interaction with isoniazid.
当将异烟肼应用于鸡胚的绒毛尿囊膜时,结果显示它会使胚胎的脆弱性增加,并且使从骨骼中用冷的1M氯化钠可提取的胶原蛋白量增加。给予吡哆醛几乎能完全逆转这些现象。异烟肼的作用与β-氨基丙腈不同,后者的作用更强,且不受吡哆醛影响;而β-氨基丙腈会导致骨骼畸形,异烟肼即使浓度为其12倍也不会产生畸形。已证明吡哆醛的醛基对于其与异烟肼的相互作用是必需的。