Michel F, Hoffenbach A, Froussard P, Langlade-Demoyen P, Kaczorek M, Kieny M P, Plata F
Laboratoire d'Immunopathologie des Rétrovirus, Institut Pasteur, Paris, France.
AIDS Res Hum Retroviruses. 1992 Apr;8(4):469-78. doi: 10.1089/aid.1992.8.469.
Cellular immunogenicity of env gp160, nef p27, and gag p55 proteins of human immunodeficiency virus type 1 (HIV-1) was studied in mice immunized with vaccinia virus recombinants. Proliferative responses of spleen cells were comparable against env gp160, nef p27, and gag p25 recombinant proteins. No specific activity was observed against gag p18 protein. Env, nef, and gag-specific T-cell lines were generated by repeated stimulation of immune spleen cells with recombinant HIV-1 proteins. They were CD4 positive, proliferative, and also cytotoxic against HIV-transfected target cells. Specificity of the T-cell response against nef and gag protein was analyzed with synthetic peptides. Peptides nef 15, nef 16, and gag AM-30 were, respectively, reactive in nef- and gag-specific proliferative and cytolytic assays. The three peptides described have a relatively conserved amino acid sequence among HIV isolates and appear broadly immunoreactive among species.
在接种痘苗病毒重组体的小鼠中研究了1型人类免疫缺陷病毒（HIV-1）的包膜糖蛋白gp160、负调控因子p27和群抗原p55蛋白的细胞免疫原性。脾细胞对包膜糖蛋白gp160、负调控因子p27和群抗原p25重组蛋白的增殖反应相当。未观察到对群抗原p18蛋白的特异性活性。通过用重组HIV-1蛋白反复刺激免疫脾细胞，产生了包膜、负调控因子和群抗原特异性T细胞系。它们是CD4阳性的、具有增殖能力的，并且对HIV转染的靶细胞也具有细胞毒性。用合成肽分析了T细胞对负调控因子和群抗原蛋白反应的特异性。肽nef 15、nef 16和群抗原AM-30分别在负调控因子和群抗原特异性增殖及溶细胞试验中具有反应性。所描述的这三种肽在HIV分离株中具有相对保守的氨基酸序列，并且在种属间表现出广泛的免疫反应性。
