Hoke R A, Giesy J P, Kreis R G
Department of Fisheries and Wildlife, Michigan State University, East Lansing 48824-1222.
Ecotoxicol Environ Saf. 1992 Jun;23(3):343-54. doi: 10.1016/0147-6513(92)90083-f.
Microtox assays with two different methods of osmotic adjustment were used to assess the toxicity of pore waters from 13 sediment samples collected from the Fox River watershed in Wisconsin. No toxicity was observed in Microtox assays osmotically adjusted with NaCl; however, 15-min EC50 values for assays osmotically adjusted with sucrose ranged from 52 to 63% pore water. Un-ionized ammonia accounted for a large part of the observed toxicity, but, based on a toxic units approach, did not account for all observed toxicity. Metals (Cu, Zn) and an unidentified compound(s) may potentially contribute to the observed effects in Microtox assays osmotically adjusted with sucrose. The use of alternative osmotic adjustment techniques in the Microtox assay is one potentially useful tool for elucidating several classes of compounds responsible for effects observed in toxicity assays.
采用两种不同渗透调节方法的微毒性试验，来评估从威斯康星州福克斯河流域采集的13个沉积物样本孔隙水的毒性。在用氯化钠进行渗透调节的微毒性试验中未观察到毒性；然而，用蔗糖进行渗透调节的试验中，15分钟半数有效浓度（EC50）值范围为孔隙水的52%至63%。未电离氨占所观察到的毒性的很大一部分，但基于毒性单位法，不能解释所有观察到的毒性。金属（铜、锌）和一种未鉴定的化合物可能对用蔗糖进行渗透调节的微毒性试验中所观察到的效应有潜在贡献。在微毒性试验中使用替代渗透调节技术是一种潜在有用的工具，可用于阐明在毒性试验中观察到的效应所涉及的几类化合物。
