用二氟二硝基苯将蛋白质抗原与红细胞偶联。
The coupling of protein antigens to erythrocytes with difluorodinitrobenzene.
作者信息
LING N R
出版信息
Immunology. 1961 Jan;4(1):49-54.
Abstract
A sensitive indirect haemagglutination technique is described which depends upon attachment of the protein antigen to the erythrocyte with difluorodinitrobenzene. The attachment of human gamma globulin to human erythrocytes has been thoroughly studied using sera containing the Rose-Waaler factor as the agglutinator. It has also been shown that horse and rabbit serum proteins and egg white may be attached to cells by the reagent. The technique is simple and sensitive but an absorption step is necessary to remove a non-specific agglutinin to cells which have been treated with the reagent.
摘要
本文描述了一种灵敏的间接血凝技术，该技术依赖于通过二氟二硝基苯将蛋白质抗原附着于红细胞。使用含有罗斯 - 瓦勒因子的血清作为凝集剂，对人γ球蛋白与人红细胞的附着进行了深入研究。还表明，马和兔血清蛋白以及蛋清也可用该试剂附着于细胞。该技术简单且灵敏，但需要一个吸收步骤来去除对用该试剂处理过的细胞的非特异性凝集素。
相似文献
1
The coupling of protein antigens to erythrocytes with difluorodinitrobenzene.
Immunology. 1961 Jan;4(1):49-54.
2
Extent of cross-linking of amino-phospholipid neighbors in the erythrocyte membrane as influenced by the concentration of difluorodinitrobenzene.
Biochem Biophys Res Commun. 1974 Nov 6;61(1):30-7. doi: 10.1016/0006-291x(74)90529-4.
3
HEMAGGLUTINATION REACTIONS OF HUMAN ERYTHROCYTES CONJUGATED COVALENTLY WITH DINITROPHENYL GROUPS.
J Immunol. 1965 Mar;94:317-22.
4
Alterations of the erythrocyte membrane caused by fluorinated dinitrobenzenes.
Experientia. 1968 Sep 15;24(9):949-50. doi: 10.1007/BF02138676.
5
[STUDIES ON THE EFFECT OF TANNIN AND FORMALIN ON HETEROANTIGENS OF HUMAN AND ANIMAL ERYTHROCYTES].
Z Immunitats Allergieforsch. 1964 Apr;126:312-24.
6
[Inhibitory action of some chemical substances on Le-a erythrocyte antigens].
Z Immun exp ther. 1961 Dec;122:368-75.
7
Effect of proteolytic enzymes on agglutinogens of human red cells.
Acta Pathol Microbiol Scand Suppl. 1961;51(Suppl 144):227-8.
8
THE RELATIVE HEAT STABILITY OF ANTIBODIES IN CHROMATOGRAPHIC FRACTIONS OF RABBIT ANTISERA TO VARIOUS ANTIGENS.
J Immunol. 1965 Jan;94:31-6.
9
Studies on the indirect hemagglutination test and hemolysis test by erythrocytes sensitized with H. pertussis antigens. 1.
Kitasato Arch Exp Med. 1953 Dec;26(4):217-29.
10
ACTINOMYCIN D EFFECT ON THE PRIMARY IMMUNE RESPONSE IN MICE: A HISTOLOGIC AND SEROLOGIC STUDY.
Lab Invest. 1965 Mar;14:272-84.
引用本文的文献
1
[ON PROTEIN-BINDING TO ERYTHROCYTES BY DINITRODIFLUOROBENZENE].
Z Hyg Infektionskr. 1963 Jul 23;149:206-9.
2
Pregnancy diagnosis by a one-stage passive haemagglutination inhibition method.
Br Med J. 1963 Apr 20;1(5337):1049-54. doi: 10.1136/bmj.1.5337.1049.
3
[X-ray sensitivity of embryonated Capillaria hepatica eggs and serum-GLDH activities and antibody titres in Mastomys natalensis infected with untreated or irradiated eggs].
Z Parasitenkd. 1981;65(1):107-16. doi: 10.1007/BF00926560.
4
Coupling of protein antigens to erythrocytes through disulfide bond formation: preparation of stable and sensitive target cells for immune hemolysis.
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2493-6. doi: 10.1073/pnas.78.4.2493.
5
Serological and biological activities of anti-Haemophilus influenzae ribosomal serum.
Infect Immun. 1981 Mar;31(3):1125-31. doi: 10.1128/iai.31.3.1125-1131.1981.
6
7
Production of different classes of immunoglobulins by individual cells in the guinea-pig.
Immunology. 1969 Mar;16(3):349-59.
8
9
10
Comparative evaluation of different fixation procedures and different coupling reagents for the demonstration of influenza virus-specific antibodies by the indirect hemagglutination test.
Med Microbiol Immunol. 1975 Dec 30;162(1):43-53. doi: 10.1007/BF02123576.
本文引用的文献
1
The terminal peptides of insulin.
Biochem J. 1949;45(5):563-74. doi: 10.1042/bj0450563.
2
The amino-acid sequence in the phenylalanyl chain of insulin. I. The identification of lower peptides from partial hydrolysates.
Biochem J. 1951 Sep;49(4):463-81. doi: 10.1042/bj0490463.
3
Studies on the immunochemistry of human low density lipoproteins utilizing an hemagglutination technique.
J Exp Med. 1959 Jul 1;110(1):113-22. doi: 10.1084/jem.110.1.113.
4
Adaptation of the antiglobulin test for use with tannic acid treated erythrocytes.
J Immunol. 1959 Apr;82(4):279-89.
5
Hemagglutination tests with Ascaris antigens.
J Immunol. 1958 May;80(5):396-9.
6
7
A passive hemagglutination reaction for psittacosis.
J Immunol. 1958 Feb;80(2):94-9.
8
Experimental evidence for the role of auto-immunization in chronic thyroiditis.
Proc R Soc Med. 1957 Nov;50(11):955-8.
9
Studies with 15N-labelled nitrogen mustards; the combination of di-2-(chloroethyl)methylamine with proteins.
Biochem J. 1957 Jul;66(3):504-15. doi: 10.1042/bj0660504.
10
Studies on phospholipids. II. 2:4-Dinitrophenyl-kephalins.
Biochem J. 1957 Jul;66(3):441-50. doi: 10.1042/bj0660441.
Zotero 插件