Kangasniemi M, Cheng C Y, Toppari J, Grima J, Stahler M, Bardin C W, Parvinen M
Department of Anatomy, University of Turku, Finland.
J Androl. 1992 May-Jun;13(3):208-13.
Production of several proteins by rat Sertoli cells is dependent on the stage of the cycle of the seminiferous epithelium. The authors have determined steady state levels and follicle-stimulating hormone responsiveness of three Sertoli cell products in culture media of rat seminiferous tubule segments at different stages of the epithelial cycle: SGP-2 (sulfated glycoprotein-2), alpha 2-macroglobulin, and testibumin. Basal SGP-2 levels were twofold higher in stages VII through VIII compared with stages XIII to I to VI (P less than 0.05). Highest basal alpha 2-macroglobulin levels were found in stages II through VIII; this was about 35% greater than in stages XIII through I of the cycle (P less than 0.05). Basal testibumin levels were twofold higher in stages II through VI compared with stages IX through XII of the cycle. Follicle-stimulating hormone had no effect on SGP-2, but by contrast it (50 mg/L) increased the level of alpha 2-macroglobulin significantly (P less than 0.05) in stages XIII through I. Follicle-stimulating hormone treatment (10 mg/L) elevated testibumin levels at each stage-pool by about 40% (P less than 0.05). The current results using staged tubular segments in vitro demonstrate cyclic basal steady-state levels of the three proteins along the seminiferous tubules and follicle-stimulating hormone regulation of alpha 2-macroglobulin and testibumin.
大鼠支持细胞产生的几种蛋白质取决于生精上皮周期的阶段。作者测定了上皮周期不同阶段大鼠生精小管段培养基中三种支持细胞产物的稳态水平和促卵泡激素反应性：SGP-2（硫酸化糖蛋白-2）、α2-巨球蛋白和睾丸白蛋白。与第XIII至I期和VI期相比，第VII至VIII期的基础SGP-2水平高出两倍（P<0.05）。在第II至VIII期发现α2-巨球蛋白的基础水平最高；这比周期的第XIII至I期高出约35%（P<0.05）。与周期的第IX至XII期相比，第II至VI期的基础睾丸白蛋白水平高出两倍。促卵泡激素对SGP-2没有影响，但相比之下，它（50mg/L）在第XIII至I期显著提高了α2-巨球蛋白的水平（P<0.05）。促卵泡激素处理（10mg/L）使每个阶段组的睾丸白蛋白水平提高了约40%（P<0.05）。目前使用体外分期管状段的结果表明，沿生精小管的三种蛋白质的基础稳态水平呈周期性变化，以及促卵泡激素对α2-巨球蛋白和睾丸白蛋白的调节作用。
