Lipman N S, Trudel L J, Murphy J C, Sahali Y
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge 02139.
Lab Anim Sci. 1992 Apr;42(2):193-7.
Freund's adjuvant and the RIBI adjuvant system were compared for their immune potentiating and toxic effects. Each adjuvant was administered with benzo(a)pyrene (BaP), a nonimmunogenic hapten, conjugated to a bovine gamma globulin (BGG) carrier protein to 10 mice intraperitoneally. Complete Freund's adjuvant was used at initial immunization, while incomplete Freund's was used for booster immunizations. Five mice were given the immunogen conjugate (BaP-BGG) in saline as a control. Antibody titers were determined by ELISA to both hapten and carrier after each of the two booster immunizations. Titers to BaP were 2- and 27-fold higher for RIBI than for Freund's after each of two booster immunizations. Titers to bGG were 119 and 12-fold higher for RIBI compared with Freund's. Titers to both immunogens were markedly less when administered in saline. Body weights were monitored in all three groups for the duration of the study. No differences were observed among the three groups. Mice from each group were euthanized at regular intervals to assess pathology. Splenic weight:body weight ratios were determined at the time of necropsy, and no differences were noted among the three groups. Granulomatous inflammatory lesions were most severe in the Freund's immunized mice, less severe in those immunized with RIBI, and least with saline. Results indicate that the RIBI system was more effective in potentiating an immune response and elicited less tissue reaction than did Freund's adjuvant with this particular immunogen.
比较了弗氏佐剂和RIBI佐剂系统的免疫增强作用和毒性作用。将每种佐剂与一种非免疫原性半抗原苯并(a)芘（BaP）结合到牛γ球蛋白（BGG）载体蛋白上，腹腔注射给10只小鼠。初次免疫时使用完全弗氏佐剂，而加强免疫时使用不完全弗氏佐剂。5只小鼠给予盐水中的免疫原缀合物（BaP-BGG）作为对照。在两次加强免疫后的每一次之后，通过ELISA测定对半抗原和载体的抗体滴度。在两次加强免疫后的每一次之后，RIBI对BaP的滴度比弗氏佐剂高2倍和27倍。与弗氏佐剂相比，RIBI对bGG的滴度高119倍和12倍。当在盐水中给药时，对两种免疫原 的滴度明显较低。在研究期间监测所有三组小鼠的体重。三组之间未观察到差异。定期对每组小鼠实施安乐死以评估病理学情况。在尸检时测定脾脏重量与体重的比值，三组之间未发现差异。肉芽肿性炎性病变在弗氏佐剂免疫的小鼠中最严重，在RIBI免疫的小鼠中较轻，在盐水组中最轻。结果表明，对于这种特定的免疫原，RIBI系统在增强免疫反应方面更有效，并且比弗氏佐剂引起的组织反应更小。
