LaPan K E, Klapper D G, Frelinger J A
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599-7290.
Hybridoma. 1992 Apr;11(2):217-23. doi: 10.1089/hyb.1992.11.217.
We report on the production and characterization of two murine peptide specific anti-major histocompatibility complex class II chain specific monoclonal antibodies. Two new mouse monoclonal antibodies reactive with two synthetic peptides corresponding to Abb amino acids (146-157) and Abs amino acids (146-157) were produced. KL295 is the mouse anti-Abb monoclonal antibody, which reacts with denatured beta chains of H-2b, H-2d, H-2p, and H-2q, but fail to react with spleen cell lysates from H-2f, H-2j, H-2k and H-2s mice. The mouse anti Abs monoclonal antibody, KL304 in contrast reacts with the denatured Class II beta chains of H-2f, H-2j, H-2k and H-2s mice, but fails to bind H-2b, H-2d, H-2p or H-2q beta chain, suggesting residues 153-155 of this molecule to be critical for the epitope. Both KL295 and KL304 bind B10.WB (H-2j) which suggests a unique epitope in this strain of mice. Neither KL295 or KL304 react with native mouse class II cell surface molecules.
我们报告了两种鼠源肽特异性抗主要组织相容性复合体II类链特异性单克隆抗体的制备及特性。制备了两种新的小鼠单克隆抗体，它们分别与对应于Abb氨基酸（146 - 157）和Abs氨基酸（146 - 157）的两种合成肽发生反应。KL295是鼠抗Abb单克隆抗体，它能与H - 2b、H - 2d、H - 2p和H - 2q的变性β链发生反应，但不与H - 2f、H - 2j、H - 2k和H - 2s小鼠的脾细胞裂解物发生反应。相比之下，鼠抗Abs单克隆抗体KL304能与H - 2f、H - 2j、H - 2k和H - 2s小鼠的变性II类β链发生反应，但不与H - 2b、H - 2d、H - 2p或H - 2q的β链结合，这表明该分子的153 - 155位残基对于表位至关重要。KL295和KL304都能与B10.WB（H - 2j）结合，这表明在该品系小鼠中存在一个独特的表位。KL295和KL304都不与天然小鼠II类细胞表面分子发生反应。
