Vazquez A M, Tormo B, Velandia A, Giscombe R, Ansotegui I, Jeddi-Tehrani M, Perez R, Mellstedt H, Biberfeld P
National Institute of Oncology, C. Habana, Cuba.
Hybridoma. 1992 Apr;11(2):245-56. doi: 10.1089/hyb.1992.11.245.
A colorectal antigen (IOR-C2) was characterized by a monoclonal antibody produced against the colon cancer cell line SW1116. By immunohistochemical staining the antigen was abundant and strongly expressed in epithelium of normal colon whereas colorectal carcinomas showed a more variable and heterogenous reactivity to the antibody (IOR-C2). Radioimmunoprecipitates of SW1116 cell homogenates showed a 160-200 kD band in SDS gels. Physicochemical characterization indicate that at least two IOR-C2 reactive sites are present on the antigen tested and that it is mainly an 0-linked glycoprotein carbohydrate chain which can also be N-linked to the protein. The expression of IOR-C2 mimics that of the colon associated antigen (CAA) and NCC-CO-450 antigen but is distinct from these with regard to its expression in carcinomas as well as its physicochemical characteristics.
一种结肠直肠抗原（IOR-C2）由针对结肠癌细胞系SW1116产生的单克隆抗体所鉴定。通过免疫组织化学染色，该抗原在正常结肠上皮中丰富且强烈表达，而结肠直肠癌对该抗体（IOR-C2）表现出更具变化性和异质性的反应。SW1116细胞匀浆的放射免疫沉淀物在SDS凝胶中显示出一条160 - 200 kD的条带。物理化学特性表明，在测试的抗原上存在至少两个IOR-C2反应位点，并且它主要是一种O-连接的糖蛋白碳水化合物链，也可以N-连接到蛋白质上。IOR-C2的表达与结肠相关抗原（CAA）和NCC-CO-450抗原的表达相似，但在癌组织中的表达及其物理化学特性方面与它们不同。
