MELLORS R C, MUNROE J S
J Exp Med. 1960 Nov 1;112(5):963-74. doi: 10.1084/jem.112.5.963.
A study has been made of the immunospecific, apple-green staining patterns imparted by fluorescent antibody to Rous virus in frozen sections of wing tumors produced in young chickens by various initiating doses of virus. Variations in the cellular site (cytoplasm, cell membrane, and nucleus), the distribution (particulate or homogeneous), and the quantity (little or much) of Rous viral antigens were seen. The per cent of sarcoma cells containing viral antigens was related to the tumor initiating dose of virus and to the infectivity titer of virus extracted from the wing tumor. With certain assumptions it was estimated that a quantity of virus approaching one tumor-producing dose (1 TD(50)) per tissue section was detectable with fluorescent antibody. Sarcoma cells containing viral antigens were identified as early as 4 days after inoculation of Rous virus. At this time viral antigens were also localized in the skeletal muscle of the inoculated wing in a characteristic focal pattern of deposition near or on the sarcolemma. This association between viral antigens and skeletal muscle fibers was observed also at 3 days after virus inoculation, when sarcoma cells were not yet demonstrable in the tissue sections. Viral antigens were detected in sarcoma cells in lung tumors of young chickens and in tumors of the chorioallantoic membrane of chick embryos.
对用不同起始剂量病毒诱发的幼鸡翼部肿瘤冷冻切片中，荧光抗体对劳斯病毒产生的免疫特异性苹果绿染色模式进行了研究。观察到劳斯病毒抗原在细胞部位（细胞质、细胞膜和细胞核）、分布（颗粒状或均匀状）以及数量（少或多）方面存在差异。含有病毒抗原的肉瘤细胞百分比与病毒的肿瘤起始剂量以及从翼部肿瘤中提取的病毒感染性滴度有关。在某些假设条件下，估计每组织切片中接近一个肿瘤产生剂量（1个TD(50)）的病毒量可用荧光抗体检测到。接种劳斯病毒后4天就可鉴定出含有病毒抗原的肉瘤细胞。此时，病毒抗原也以特征性的局灶性沉积模式定位于接种翼部的骨骼肌中，靠近或位于肌膜上。在接种病毒3天后，当组织切片中尚未显示出肉瘤细胞时，也观察到病毒抗原与骨骼肌纤维之间的这种关联。在幼鸡肺肿瘤和鸡胚绒毛尿囊膜肿瘤的肉瘤细胞中检测到了病毒抗原。
