Mellon D, Alones V, Lawrence M D
Department of Biology, University of Virginia, Charlottesville 22903.
J Comp Neurol. 1992 Jul 1;321(1):93-111. doi: 10.1002/cne.903210109.
Golgi impregnation and neurobiotin injection were used to examine details of the neural pathways in the olfactory system of the freshwater crayfish, Procambarus clarkii. Deutocerebral projection neurons (globuli cells) were directly injected with neurobiotin. These neurons have dendritic arborizations in the ipsilateral olfactory and accessory lobes, and they project axons to the lateral protocerebrum, where they terminate in microglomeruli of the hemi-ellipsoid body. The axons of the deutocerebral projection neurons are readily impregnated by Golgi procedures, and they terminate as an expanded membranous knot about 5 microns in diameter. Electron microscopy on Golgi-stained terminals has revealed that each knot makes several hundred synapses with small spine-like or shaft-like processes of postsynaptic neurons. Injection of neurobiotin into local interneurons of the hemi-ellipsoid body and subsequent examination of stained preparations with the electron microscope reveals that these cells are a major postsynaptic target of the deutocerebral projection neurons. Furthermore, the local interneurons make extensive efferent synaptic connections with unidentified neurons in the terminal medulla.
采用高尔基染色法和神经生物素注射法来研究克氏原螯虾嗅觉系统中神经通路的细节。对中脑投射神经元（小球细胞）直接注射神经生物素。这些神经元在同侧嗅觉叶和副叶中有树突分支，并且它们将轴突投射到外侧前脑，在那里它们终止于半椭球体的微小球体中。中脑投射神经元的轴突很容易被高尔基染色法浸染，并且它们终止于一个直径约5微米的膨大膜状小结。对高尔基染色的终末进行电子显微镜观察发现，每个小结与突触后神经元的小棘状或轴状突起形成数百个突触。将神经生物素注射到半椭球体的局部中间神经元中，随后用电子显微镜检查染色制剂，结果显示这些细胞是中脑投射神经元的主要突触后靶标。此外，局部中间神经元与终末髓质中未鉴定的神经元形成广泛的传出突触连接。
