Kahan A, Hamzaoui K, Ayed K
Unité 283 INSERM, Cochin Hospital, Paris, France.
J Rheumatol. 1992 May;19(5):742-6.
We assessed T cell subpopulations using 2-color flow cytometry with phycoerythrin conjugated anti-CD45RA and anti-CD29 and fluorescein conjugated anti-CD4 and anti-CD8 monoclonal antibodies, on peripheral blood lymphocytes from 19 patients with Behçet's disease (BD) and 18 healthy control subjects. The percentage of CD4+ cells was significantly lower in patients with BD (34 +/- 2%) than in control subjects (46 +/- 3%) (p less than 0.001). Among CD4+ cells, the percentage of suppressor-inducer (CD4+CD45RA+) cells was significantly lower in patients with BD (31 +/- 4%) than in control subjects (45 +/- 2%) (p less than 0.01), while the percentages of helper-inducer (CD4+CD29+) cells were similar in patients and controls. The percentage of CD8+ cells was significantly higher in patients with BD (36 +/- 2%) than in control subjects (26 +/- 2%) (p less than 0.001) involving both CD45RA+ and CD29+ subpopulations. Within CD4+ cells, the percentage of suppressor-inducer (CD4+CD45RA+) cells was significantly decreased in patients with active BD (26 +/- 3%) compared with control subjects (45 +/- 2%) (p less than 0.001), whereas in patients with inactive BD the difference was statistically insignificant. Our results suggest that the defective suppressive function in patients with active BD may be related to the decreased suppressor-inducer subpopulation (CD4+CD45RA+).
我们采用双色流式细胞术，使用藻红蛋白偶联的抗CD45RA和抗CD29以及异硫氰酸荧光素偶联的抗CD4和抗CD8单克隆抗体，对19例白塞病（BD）患者和18名健康对照者的外周血淋巴细胞进行T细胞亚群评估。BD患者中CD4+细胞的百分比（34±2%）显著低于对照者（46±3%）（p<0.001）。在CD4+细胞中，BD患者中抑制诱导细胞（CD4+CD45RA+）的百分比（31±4%）显著低于对照者（45±2%）（p<0.01），而辅助诱导细胞（CD4+CD29+）的百分比在患者和对照者中相似。BD患者中CD8+细胞的百分比（36±2%）显著高于对照者（26±2%）（p<0.001），涉及CD45RA+和CD29+亚群。在CD4+细胞中，活动期BD患者抑制诱导细胞（CD4+CD45RA+）的百分比（26±3%）与对照者（45±2%）相比显著降低（p<0.001），而在非活动期BD患者中差异无统计学意义。我们的结果表明，活动期BD患者的抑制功能缺陷可能与抑制诱导亚群（CD4+CD45RA+）减少有关。
