Sohen S, Romain P L, Rothstein D M, Yamane T, Tanaka S, Anderson P, Schlossman S F, Morimoto C
Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School.
J Rheumatol. 1991 Nov;18(11):1649-54.
We analyzed the cell surface phenotype of CD8+ cells in both peripheral blood and synovial fluid (SF) of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Utilizing the monoclonal antibodies anti-CD45RA, anti-CD29 and anti-S6F1-, one can define both suppressor effector (CD45RA+CD29-S6F1-) and killer effector (CD45RA-CD29+S6F1+) cells within the CD8 population. In patients with OA, normal proportions of CD8+CD45RA+, CD8+CD29+ and CD8+S6F1+ cells were found in both peripheral blood and SF. The peripheral blood of patients with RA, in contrast, showed a decreased percentage of CD8+CD45RA+ cells (13.4 +/- 2.6) (p less than 0.05), but a normal percentage of CD8+CD29+ and CD8+S6F1+ cells. In the SF of patients with RA, we observed a more dramatic decrease in CD8+CD45RA+ suppressor effector cells (6.4 +/- 5.0) (p less than 0.001), a significant increase in killer effector cells as measured by both CD8 + CD29+ (35.5 +/- 9.9) (p less than 0.001) and CD8 + S6F1+ cells (28.2 +/- 11.4) (p less than 0.01). These changes may contribute to the immunologic abnormalities previously noted in this disease and may provide some insight into the pathophysiologic mechanisms of RA.
我们分析了类风湿性关节炎（RA）和骨关节炎（OA）患者外周血及滑液（SF）中CD8 + 细胞的细胞表面表型。利用抗CD45RA、抗CD29和抗S6F1 - 单克隆抗体，可在CD8群体中界定抑制效应细胞（CD45RA + CD29 - S6F1 - ）和杀伤效应细胞（CD45RA - CD29 + S6F1 + ）。在OA患者中，外周血和滑液中CD8 + CD45RA + 、CD8 + CD29 + 和CD8 + S6F1 + 细胞比例正常。相比之下，RA患者外周血中CD8 + CD45RA + 细胞百分比降低（13.4±2.6）（p＜0.05），而CD8 + CD29 + 和CD8 + S6F1 + 细胞百分比正常。在RA患者的滑液中，我们观察到CD8 + CD45RA + 抑制效应细胞显著减少（6.4±5.0）（p＜0.001），通过CD8 + CD29 + （35.5±9.9）（p＜0.001）和CD8 + S6F1 + 细胞（28.2±11.4）（p＜0.01）检测到杀伤效应细胞显著增加。这些变化可能导致了此前该疾病中所发现的免疫异常，并可能为RA的病理生理机制提供一些见解。
