Kligerman A D, Allen J W, Bryant M F, Campbell J A, Collins B W, Doerr C L, Erexson G L, Kwanyuen P, Morgan D L
U.S. Environmental Protection Agency, National Institute of Environmental Health Sciences, Research Triangle Park, NC.
Mutat Res. 1992 Jul;280(1):35-43. doi: 10.1016/0165-1218(92)90016-s.
The data for the in vivo genotoxicity of styrene (STY) are equivocal. To evaluate the clastogenicity and sister-chromatid exchange (SCE)-inducing potential of STY in vivo under carefully controlled conditions, B6C3F1 female mice were exposed by inhalation for 6 h/day for 14 consecutive days to either 0, 125, 250 or 500 ppm STY. One day after the final exposure, peripheral blood, spleen, and lungs were removed and cells were cultured for the analysis of micronucleus (MN) induction using the cytochalasin B-block method, chromosome breakage, and SCE induction. Peripheral blood smears were also made for scoring MN in erythrocytes. There was a significant concentration-related elevation of SCE frequency in lymphocytes from the spleen and the peripheral blood as well as in cells from the lung. However, no statistically significant concentration-related increases were found in the frequency of chromosome aberrations in the cultured splenocytes or lung cells, and no significant increases in MN frequencies were observed in binucleated splenocytes or normochromatic erythrocytes in peripheral blood smears.
苯乙烯（STY）体内遗传毒性的数据并不明确。为了在严格控制的条件下评估STY在体内的致断裂性和诱导姐妹染色单体交换（SCE）的潜力，将B6C3F1雌性小鼠连续14天每天吸入暴露6小时，暴露浓度分别为0、125、250或500 ppm的STY。在最后一次暴露后一天，取出外周血、脾脏和肺，培养细胞，采用细胞松弛素B阻断法分析微核（MN）诱导情况、染色体断裂情况以及SCE诱导情况。还制作了外周血涂片以对红细胞中的MN进行评分。脾脏和外周血淋巴细胞以及肺细胞中的SCE频率出现了与浓度相关的显著升高。然而，在培养的脾细胞或肺细胞中，染色体畸变频率未发现与浓度相关的统计学显著增加，在外周血涂片的双核脾细胞或正染红细胞中也未观察到MN频率的显著增加。
