Wilson L A, Thompson J J, Ohkawa S, Scott D W
Department of Microbiology, Immunology, Louisiana State University Medical Center, New Orleans.
Viral Immunol. 1992 Summer;5(2):113-22. doi: 10.1089/vim.1992.5.113.
Pretreatment of mice iv with syngeneic spleen cells modified with soluble HSV envelope antigens induced an anti-HSV antibody hyporesponsiveness following challenge with infectious virus. The epitope density on the HSV-modified spleen cells was quantitated using a photon-counting spectrofluorimeter so that observed immunological results could be correlated with the HSV antigen dose on the splenocytes. The degree of anti-HSV antibody hyporesponsiveness was found to be related to the epitope density on the HSV-modified spleen cells, but not the number of modified cells used in the pretreatment over the 16-fold range tested. Anti-HSV antibody hyporesponsiveness was induced if 7, but not 3, days had elapsed between pretreatment and challenge. This antibody hyporesponsiveness could be adoptively transferred with T cells. Only mice that had induced an anti-HSV antibody hyporesponsiveness following pretreatment with HSV-modified splenocytes were able to survive an LD50 challenge with infectious virus.
用可溶性单纯疱疹病毒（HSV）包膜抗原修饰的同基因脾细胞对小鼠进行静脉内预处理后，在用感染性病毒攻击时会诱导抗HSV抗体低反应性。使用光子计数荧光分光光度计对HSV修饰的脾细胞上的表位密度进行定量，以便将观察到的免疫结果与脾细胞上的HSV抗原剂量相关联。发现抗HSV抗体低反应性的程度与HSV修饰的脾细胞上的表位密度有关，但与预处理中使用的修饰细胞数量在测试的16倍范围内无关。如果预处理和攻击之间间隔7天而非3天，则会诱导抗HSV抗体低反应性。这种抗体低反应性可以通过T细胞进行过继转移。只有在用HSV修饰的脾细胞预处理后诱导了抗HSV抗体低反应性的小鼠才能在感染性病毒的半数致死剂量攻击中存活下来。
