Hoefert L L, McCreight J D, Christie R D
USDA, Agricultural Research Station, Salinas, CA 93905.
Biotech Histochem. 1992 Jan;67(1):40-4. doi: 10.3109/10520299209110004.
Plant virus inclusion bodies can be stained specifically with established staining methods for light microscopy. The procedure can be augmented by a short microwave treatment to provide better staining intensity and reduced staining time. The method is useful for preliminary sampling prior to collection for electron microscopy and for plant pathologists, plant breeders, and diagnosticians as a rapid means of plant virus characterization.
植物病毒包涵体可以用已确立的光学显微镜染色方法进行特异性染色。该程序可以通过短时间的微波处理得到增强,以提供更好的染色强度并缩短染色时间。该方法对于电子显微镜采集前的初步采样很有用,并且对于植物病理学家、植物育种者和诊断人员来说,是一种快速鉴定植物病毒的方法。