Kutscher C L
Behavioral Neuroscience Laboratory Syracuse University, NY 13244.
Brain Res Bull. 1992 Apr;28(4):519-27. doi: 10.1016/0361-9230(92)90098-i.
Rats were given a single gavage of trimethyltin chloride (TMT) providing a dose of 0, 4.3, or 6.7 mg/kg of alkyltin. Gross changes in brain structures were quantified and analyzed statistically. Behavioral and functional measures were taken to verify efficacy of TMT dose. The high dose produced transient weight loss and seizures. In the fourth week after gavage, the high dose produced hyperactivity in the residential maze and activity wheel. High and low TMT doses decreased auditory startle responsiveness. Estrus cycle was normal in all groups. Brains were sectioned and stained with the Timm stain which delimited subregions of hippocampus and connected structures and also revealed mossy fibers. Linear and areal measures were made at three positions along the septotemporal axis of Ammon's horn. The low dose produced reductions in size in a few isolated subareas of the brain. The high dose produced, at the three planes studied, extensive (15-40%) loss of tissue in Ammon's horn and structures to which Ammon's horn is interconnected--subiculum, entorhinal cortex, dentate gyrus, hilus, CA3, and CA1 region. Neocortex and caudate-putamen were unaffected. These findings suggest that a single TMT gavage may disrupt brain structures important to linking neocortex with subcortex via structures in the hippocampal region.
给大鼠单次灌胃氯化三甲基锡（TMT），剂量分别为0、4.3或6.7毫克/千克烷基锡。对脑结构的大体变化进行量化并进行统计学分析。采取行为和功能测量来验证TMT剂量的效果。高剂量导致短暂体重减轻和癫痫发作。灌胃后第四周，高剂量在居住迷宫和活动轮中产生多动。高剂量和低剂量的TMT均降低听觉惊吓反应性。所有组的发情周期均正常。将大脑切片并用Timm染色，该染色界定了海马体的亚区域和相连结构，还显示了苔藓纤维。在沿海马角的隔颞轴的三个位置进行线性和面积测量。低剂量导致大脑中一些孤立的亚区域尺寸减小。在研究的三个平面上，高剂量导致海马角及其相互连接的结构——下托、内嗅皮质、齿状回、海马hilus、CA3和CA1区域——出现广泛（15 - 40%）的组织损失。新皮质和尾状核 - 壳核未受影响。这些发现表明，单次TMT灌胃可能会破坏通过海马区域的结构将新皮质与皮质下结构连接起来的重要脑结构。
